欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报

• 研究论文 • 上一篇    下一篇

虎斑乌贼的胚胎耗氧率

王鹏帅,蒋霞敏*,阮鹏,彭瑞冰,江茂旺,韩庆喜   

  1. (宁波大学海洋学院, 浙江宁波 315211)
  • 出版日期:2016-07-18

Oxygen consumption rate of Sepia pharaonis embryos.

WANG Peng-shuai, JIANG Xia-min*, RUAN Peng, PENG Rui-bing, JIANG Mao-wang, HAN Qing-xi   

  1. (School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China).
  • Online:2016-07-18

摘要:

为了探究虎斑乌贼胚胎不同发育时期的耗氧率变化和几种生态因子对胚胎发育过程耗氧率的影响,试验采用封闭静水装置,对不同发育时期(12期)的耗氧率进行测定,并研究不同盐度(21、24、27、30、33)、温度(18、21、24、27、30 ℃)和pH(7.0、7.5、8.0、8.5、9.0)对胚胎4个主要发育时期(受精卵期、原肠胚期、器官形成期和内骨骼形成期)耗氧率的影响.结果表明: 胚胎各个发育时期耗氧率不同,随着发育的进程而增大,受精卵期为0.082 mg·(100 eggs)-1·h-1,而到原肠胚期的耗氧率显著升高,为0.279 mg·(100 eggs)-1·h-1,到孵化期时,耗氧率达到1.367 mg·(100 eggs)-1·h-1;盐度对器官形成期和内骨骼形成期的耗氧率均有显著影响(P<0.05),对受精卵期和原肠胚期影响不显著(P>0.05),当盐度为30时,4个发育时期耗氧率均达到最大值,分别为0.082、0.200、0.768和1.301 mg·(100 eggs)-1·h-1;温度对原肠胚期、器官形成期和内骨骼形成期的耗氧率有显著影响(P<0.05),对受精卵期无显著性影响(P>0.05),在27 ℃时,胚胎4个发育时期均达到最大值,分别为0.082、0.286、0.806和1.338 mg·(100 eggs)-1·h-1;而pH对4个发育时期的耗氧率均无显著性影响(P>0.05),受精卵期在pH 8.0时达到最大值,为0.116 mg·(100 eggs)-1·h-1,原肠胚期、器官形成期、内骨骼形成期在pH 8.5时达到最大值,分别为0.281 、0.799和1.130 mg·(100 eggs)-1·h-1.
 

关键词: 虎斑乌贼, 生态因子, 胚胎, 耗氧率

Abstract: This research was conducted to unravel the variation of oxygen consumption rate during different developmental stages and the effects of different ecological factors on embryonic oxygen consumption rate of Sepia pharaonis. The oxygen consumption rates were measured at twelve developmental stages by the sealed volumetric flasks, and four embryonic developmental periods (oosperm, gastrula, the formation of organization, endoskeleton) were selected under various ecological conditions, such as salinity (21, 24, 27, 30, 33), water temperature (18, 21, 24, 27, 30 ℃) and pH (7.0, 7.5, 8.0, 8.5, 9.0). The results showed that the oxygen consumption rate rose along with the developmental progress, and distinctly differed from each other. The oxygen consumption rate was 0.082 mg·(100 eggs)-1·h-1 during oosperm period, and rose to 0.279 mg·(100 eggs)-1·h-1 during gastrula period, which was significantly higher than that of blastula period. Finally, the oxygen consumption rate rose to 1.367 mg·(100 eggs)-1·h-1 during hatching period.  The salinity showed a significant effect on oxygen consumption rate during the formation of organization and endoskeleton formation stage (P<0.05), but no significant effect during oosperm and gastrula periods (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of salinity, and reached the highest values \[0.082, 0.200, 0.768 and 1.301 mg·(100 eggs)-1·h-1, respectively\] at salinity 30. The water temperature had a significant effect on the embryo oxygen consumption rates of gastrula, and the formation of organization and endoskeleton formation stage (P<0.05), with the exception of oosperm (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of temperature, and reached the highest values at 27 ℃ \[0.082, 0.286, 0.806 and 1.338 mg·(100 eggs)-1·h-1, respectively\]. The pH had no significant effect on the oxygen consumption rates of four embryonic stages (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of pH. The oxygen consumption rates of gastrula, the formation of organization, endoskeleton reached the according highest values \[0.281, 0.799 and 1.130 mg·(100 eggs)-1·h-1\] at pH 8.5, but that during oosperm period occurred at pH 8.0 \[0.116 mg·(100 eggs)-1·h-1\].

Key words: Sepia pharaonis, ecological factors, oxygen consumption rate, embryo