Soil nitrogen content and enzyme activities in rhizosphere and non-rhizosphere of summer maize under different nitrogen application rates.

doi:10.13287/j.1001-9332.201606.031

Abstract

Abstract: A field experiment was conducted on fluvo-aquic soil in the North China Plain to study the effects of nitrogen application rate on soil nitrogen contents and enzyme activities in rhizosphere and non-rhizosphere of summer maize. The results showed that the soil enzyme activities under different nitrogen application rates showed similar seasonal patterns. In comparison to no nitrogen ferti-lizer treatment, all nitrogen application treatments significantly increased NO₃^--N contents in rhizosphere and non-rhizosphere soils, NH₄⁺-N content in rhizosphere soil and the activities of β-N-acetylglucosaminidase, β-glucosidase, β-xylosidase and Cellobiohyrolase. During the whole summer maize growing season, the NO₃^--N content in non-rhizosphere soil was significantly higher than that in rhizosphere soil. The NH₄⁺-N content in non-rhizosphere soil was also significantly higher than that in rhizosphere soil at filling stage but significantly lower at seedling and maturity stages. Furthermore, soil enzyme activities in rhizosphere soil were significantly higher than those in non-rhizosphere soil. Effect of nitrogen application on soil organic carbon content was not significant. Soil total nitrogen content increased significantly when the nitrogen application rate was 0-180 kg·hm^-2 but decreased significantly when the rate was higher than 180 kg·hm^-2. Generally, a proper rate of nitrogen fertilizer application could significantly increase soil enzyme activities and total nitrogen content, and then improve soil biochemistry properties.

LIANG Guo-peng, Houssou A. Albert, WU Hui-jun, WU Xue-ping, CAI Dian-xiong, GAO Li-li, LI Jing, WANG Bi-sheng, LI Sheng-ping. Soil nitrogen content and enzyme activities in rhizosphere and non-rhizosphere of summer maize under different nitrogen application rates.[J]. Chinese Journal of Applied Ecology, 2016, 27(6): 1917-1924.

References

[1] Gianfreda L, Sannino F, Vtoante A. Pesticide effects on the activity of tree, immobilized and invertase. Soil Bio-logy and Biochemistry, 1995, 27: 1201-1208
[2] Fang F (方昉), Wu C-Z (吴承祯), Hong W (洪伟), et al. Study on the relationship between rhizospheric soil enzyme and microbe in different plants. Subtropical Agriculture Research (亚热带农业研究), 2007, 3(3): 209-215 (in Chinese)
[3] Tian Y-H (田幼华), Lv G-H (吕光辉), Yang X-D (杨晓东), et al. Influence of water and saline stress on rhizosphere soil enzyme activities for arid region plants. Journal of Arid Land Resource and Environment (干旱区资源与环境), 2012, 26(3): 158-163 (in Chinese)
[4] Li YT, Rouland C, Benedetti M, et al. Microbial biomass, enzyme and mineralization activity in relation to soil organic C, N and P turnover influenced by acid metal stress. Soil Biology and Biochemistry, 2009, 41: 969-977
[5] Gispert M, Emran M, Pardini G, et al. The impact of land management and abandonment on soil enzymatic activity, glomalin content and aggregate stability. Geoderma, 2013, 202/203: 51-61
[6] Ye J-Y (叶家颖), Ma C-H (马承豪), Yang A-P (杨爱平), et al. A study on enzyme activity of persimmon root range soil. Journal of Guangxi Normal University (Natural Science) (广西师范大学学报:自然科学版), 2000, 18(4): 82-86 (in Chinese)
[7] Lu H-F (陆海飞), Zheng J-W (郑金伟), Yu X-C (余喜初), et al. Microbial community diversity and enzyme activity of red paddy soil under long-term combined inorganic-organic fertilization. Plant Nutrition and Ferti-lizer Science (植物营养与肥料学报), 2015, 21(3): 632-643 (in Chinese)
[8] Guo T-C (郭天财), Song X (宋晓), Ma D-Y (马冬云), et al. Effect of nitrogen application rate on soil enzyme activities in wheat rhizosphere. Chinese Journal of Applied Ecology (应用生态学报), 2008, 19(1): 110-114 (in Chinese)
[9] Morris RA, Garrity DP. Resource capture and utilization in intercropping: Non-nitrogen nutrients. Field Crops Research, 1993, 34: 319-334
[10] Li J-H (李俊华), Shen Q-R (沈其荣), Zhu G-X (褚贵新), et al. Effects of application amino acid fertilizer on soil enzyme activity and available nutrients in cotton rhizosphere and bulk soils. Soils (土壤), 2011, 43(2): 277-284 (in Chinese)
[11] Chen H-J (陈闳竣), Li C-H (李传涵). Comparison in enzyme activities between the soils in rhizosphere and non-rhizosphere of Cunninghamia lanceolata. Scientia Silvae Sinicae (林业科学), 1994, 30(2): 170-175 (in Chinese)
[12] Yun P (云鹏), Gao X (高翔), Chen L (陈磊), et al. Plant nitrogen utilization and soil nitrogen status in rhizosphere of maize as affected by various nitrogen rates in wheat-maize rotation system. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 2010, 16(3): 567-574 (in Chinese)
[13] Yi Z-X (易镇邪), Wang P (王璞), Shen L-X (申丽霞), et al. Effects of different types of nitrogen fertilizer on nitrogen accumulation,translocation and nitrogen fertili-zer utilization in summer maize. Acta Agronomica Sinica (作物学报), 2006, 32(5): 772-778 (in Chinese)
[14] Ju X-T (巨晓棠), Liu X-J (刘学军), Zhang F-S (张福锁), et al. Study on effect of nitrogen fertilizer and nitrogen balance in winter wheat and summer maize rotation system. Scientia Agricultura Sinica (中国农业科学), 2002, 35(11): 1361-1368 (in Chinese)
[15] Zhang S-L (张绍林), Zhu Z-L (朱兆良), Xu Y-H (徐银华), et al. Optimal application rate of nitrogen fertilizer for rice and wheat in Taihu Lake region. Soils (土壤), 1988, 42(1): 5-9 (in Chinese)
[16] Su J-Q (苏洁琼), Li X-R (李新荣), Bao J-T (包婧婷). Effect of nitrogen addition on soil physico-chemical properties and enzyme activities in desertified steppe. Chinese Journal of Applied Ecology (应用生态学报), 2014, 25(3): 664-670 (in Chinese)
[17] Su D (苏丹), Zhang K (张凯), Chen F-L (陈法霖), et al. Effects of nitrogen application on soil microbial community composition and function in Eucalyptus plantations with different soil organic carbon levels. Ecology and Environmental Sciences (生态环境学报), 2014, 23(3): 423-429 (in Chinese)
[18] Xia X (夏雪), Gu J (谷洁), Che S-G (车升国), et al. Effects of nitrogen application rates on microbial community and enzyme activities in Lou soil. Scientia Agricultura Sinica (中国农业科学), 2011, 44(8): 1618-1627 (in Chinese)
[19] Marx MC, Wood M, Jarvis SC. A microplate fluorime-tric assay for the study of enzyme diversity in soils. Soil Biology and Biochemistry, 2001, 33: 1633-1640
[20] De Forest JL. The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and L-DOPA. Soil Biology and Biochemistry, 2009, 41: 1180-1186
[21] Allison SD, Vitousek PM. Responses of extracellular enzymes to simple and complex nutrient inputs. Soil Biology and Biochemistry, 2005, 37: 937-944
[22] Allison SD, Czimczik CI, Treseder KK. Microbial acti-vity and soil respiration under nitrogen addition in Alaskan boreal forest. Global Change Biology, 2008, 14: 1156-1168
[23] Zaidi A, Khan MS. Interactive effect of rhizotrophic microorganisms on growth, yield, and nutrient uptake of wheat. Journal of Plant Nutrition, 2005, 28: 2079-2092
[24] Ma Z-B (马宗斌), Xiong S-P (熊淑萍), He J-G (何建国), et al. Effects of nitrogen forms on rhizosphere microorganisms and soil enzyme activity under cultivation of contrasting wheat cultivars during booting and grain filling period. Acta Ecologica Sinica (生态学报), 2008, 28(4): 1544-1551 (in Chinese)
[25] Ai C, Liang GQ, Sun JW, et al. Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil. Soil Biology and Biochemistry, 2013, 57: 30-42
[26] Tian J, Lu SH, Fan MS, et al. Integrated management systems and N fertilization: Effect on soil organic matter in rice-rapeseed rotation. Plant and Soil, 2013, 372: 53-63
[27] He YT, Zhang WJ, Xu MG, et al. Long-term combined chemical and manure fertilizations increase soil organic carbon and total nitrogen in aggregate fractions at three typical cropland soils in China. Science of the Total Environment, 2015, 532: 635-644
[28] Jung JY, Lal R, Jastrow JD, et al. Nitrogenous fertilizer effects on soil structural properties under switch grass. Agriculture, Ecosystems and Environment, 2011, 141: 215-220
[29] Xie J-Y (谢驾阳), Wang Z-H (王朝辉), Li S-X (李生秀). Effects of nitrogen fertilizer application on soil organic carbon and nitrogen accumulation and mineralization under different cultivations in northwest dryland. Journal of Northwest A&F University (Natural Science) (西北农林科技大学学报:自然科学版), 2009, 37(11): 187-200 (in Chinese)
[30] Li X-H (李小涵), Li F-C (李富翠), Liu J-S (刘金山), et al. Changes of different carbon fractions caused by long-term N fertilization in dryland soil of the Loess Plateau. Scientia Agricultura Sinica (中国农业科学), 2014, 47(14): 2795-2803 (in Chinese)
[31] Yu H-L (俞华林), Zhang E-H (张恩和), Wang Q (王琦), et al. Effects of irrigation and N supply levels on soil organic carbon,total nitrogen and grain yield of spring wheat on no-tillage farmland with standing stubble. Acta Prataculturae Sinica (草业学报), 2013, 22(3): 227-233 (in Chinese)
[32] Li W-X (李文西), Lu J-W (鲁剑巍), Lu J-M (鲁君明), et al. Effect of N application on yield of forage grass and the accumulation of N and C under a sudangrass/ryegrass rotation. Acta Prataculturae Sinica (草业学报), 2011, 20(1): 55-61 (in Chinese)
[33] Meng Y-L (孟亚利), Wang L-G (王立国), Zhou Z-G (周治国), et al. Dynamics of soil enzyme activity and nutrient content in intercropped cotton rhizosphere and non-rhizosphere. Chinese Journal of Applied Ecology (应用生态学报), 2005, 16(11): 2076-2080 (in Chinese)
[34] Li T-Q (李廷强), Zhu E (朱恩), Yang X-E (杨肖娥), et al. Studies on soil enzyme activity in rhizosphere of hyperaccumulator Sedum alfredii Hance. Journal of Soil and Water Conservation (水土保持学报), 2007, 21(3): 112-117 (in Chinese)
[35] Pathan SI, Ceccherini MT, Pietramellara G, et al. Enzyme activity and microbial community structure in the rhizosphere of two maize lines differing in N use efficiency. Plant and Soil, 2015, 387: 413-424
[36] Hinsinger P, Gobran GR, Gregory PJ, et al. Rhizosphere geometry and heterogeneity arising from root-mediated physical and chemical processes. New Phytologist, 2005, 168: 293-303
[37] Ai C, Liang GQ, Sun JW, et al. Responses of extracellular enzyme activities and microbial community in both the rhizosphere and bulk soil to long-term fertilization practices in a fluvo-aquic soil. Geoderma, 2012, 173: 330-338
[38] Li X-Y (李晓月), Zheng X-F (郑险峰), Zhou J-B (周建斌). Contents and characteristic of organic carbon and nitrogen in wheat rhizosphere with different soil textures. Chinese Journal of Soil Science (土壤通报), 2012, 43(3): 610-613 (in Chinese)
[39] Dong WY, Zhang XY, Dai XQ, et al. Changes in soil microbial community composition in response to fertilization of paddy soils in subtropical China. Applied Soil Ecology, 2014, 84: 140-147
[40] Zhan Y-Y (詹媛媛), Xue Z-Y (薛梓瑜), Ren W (任伟), et al. Characteristics of nitrogen content between rhizosphere and bulk soil under seven shrubs in arid desert area of China. Acta Ecologica Sinica (生态学报), 2009, 29(1): 59-66 (in Chinese)
[41] Meng Y-L (孟亚利), Wang L-G (王立国), Zhou Z-G (周治国), et al. Effect of the composite root population of wheat-cotton double cropping system on soil enzyme activity and soil nutrient content at the cotton rhizosphere and non-rhizosphere zones. Scientia Agricultura Sinica (中国农业科学), 2005, 38(5): 904-910 (in Chinese)