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Chinese Journal of Applied Ecology ›› 2017, Vol. 28 ›› Issue (10): 3351-3359.doi: 10.13287/j.1001-9332.201710.036

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Effects of lime-ammonium bicarbonate fumigation and biofertilizer application on Fusarium wilt and biomass of continuous cropping cucumber and watermelon.

SHEN Zong-zhuan1, SUN Li1, WANG Dong-sheng1,2, LYU Na-na1, XUE Chao1, LI Rong1*, SHEN Qi-rong1   

  1. 1. College of Resources and Environmental Sciences/Jiangsu Province Key Laboratory for Organic Solid Waste Utilization/Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing 210095, China;
    2. Nanjing Institute of Vegetable Science, Nanjing 210042, China
  • Received:2017-04-11 Revised:2017-07-14 Online:2017-10-18 Published:2017-10-18
  • Supported by:

    This work was supported by the National Key Basic Research Program of China (2015CB150500), the Fundamental Research Funds for the Central Universities (KJQN201746), the Special Fund for Argo-scientific Research in the Public Interest of Ministry of Agriculture, China (201503110), the National Key Research and Development Program (2016YFD0800605, 2016YFE0101100), the National Natural Science Foundation of China (31601836), the Science and Technology Planning Project of Guangdong Pro-vince (2016B020202006), and the Postdoctoral Science Foundation of China (2016M590469).

Abstract: In this study, the population size of soil microbes was determined using plate counting method after the application of lime-ammonium bicarbonate and ammonium bicarbonate fumigation. In addition, biofertilizer was applied after soil fumigation and population of Fusarium oxysporum, Fusarium wilt disease control efficiency and plant biomass were determined in the cucumber and watermelon continuous cropping soil. The results showed that the population of F. oxysporum in cucumber mono-cropped soil fumigated with lime-ammonium bicarbonate or ammonium bicarbonate was decreased by 95.4% and 71.4%, while that in watermelon mono-cropped soil was decreased by 87.3% and 61.2%, respectively compared with non-fumigated control (CK). Furthermore, the greenhouse experiment showed that biofertilizer application, soil fumigation and crop type showed significant effects on the number of soil F. oxysporum, Fusarium wilt disease incidence, disease control efficiency and plant biomass based on multivariate analysis of variance. In the lime-ammonium bicarbonate fumigated soil amended with biofertilizer (LFB), significant reductions in the numbers of F. oxysporum and Fusarium wilt disease incidence were observed in both cucumber and watermelon cropped soil compared to non-fumigated control soil applied with organic fertilizer. The disease control rate was 91.9% and 92.5% for cucumber and watermelon, respectively. Moreover, LFB also significantly increased the plant height, stem diameter, leaf SPAD, and dry biomass for cucumber and watermelon. It was indicated that biofertilizer application after lime-ammonium bicarbonate fumigation could effectively reduce the abundance of F. oxysporum in soil, control Fusarium wilt disease and improve plant biomass in cucumber and watermelon mono-cropping systems.