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Chinese Journal of Applied Ecology ›› 2020, Vol. 31 ›› Issue (6): 1963-1970.doi: 10.13287/j.1001-9332.202006.033

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Influencing factors of phosphate solubilizing capacity of Aspergillus niger and optimization of its culture condition

SUN Ran1, ZHANG Su1, WU Chen-lin1, LI Zhu1*, XIAO Yang2   

  1. 1College of Life Science, Guizhou University/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guiyang 550025, China;
    2Institute of Products Detection & Determination of Guizhou Province, Guiyang 550003, China
  • Received:2019-10-22 Online:2020-06-15 Published:2020-06-15
  • Contact: * E-mail: zhuliluck@163.com
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (31660533, 31460486), the Science and Technology Project of Guizhou Province (5613), the Open Fund Project of Guizhou Province Domestic First-Class Discipline Construction in Biology (GNYL[2017]009FX3KT20), and the Agricultural Project of Guizhou Science and Technology Department (3033).

Abstract: In this study, we investigated factors affecting the phosphate solubilizing capacity of Aspergillus niger. A strain named A. niger Xj-2 was isolated by single-spore isolation, with phosphate solubilizing capacity of 539.90 mg·L-1 in liquid medium. The simulated process of fermentation kinetics showed that phosphate concentration reached equilibrium after 4 d cultivation, indicating that the optimal fermentation time was around 4 d. Phosphate solubilization capacity of strain Xj-2 in different phosphorus-source culture media followed the order: calcium phosphate (539.90 mg·L-1)>zinc phosphate (238.45 mg·L-1)>ferric phosphate (182.64 mg·L-1)>phosphate rock power (71.80 mg·L-1) > aluminum phosphate (24.40 mg·L-1). We further examined the optimal condition of strain Xj-2 for phosphate solubilization by response surface optimization combined with single factor experiments. Results showed that the influence of various factors on the phosphate solubilizing capacity of strain Xj-2 was in the order: carbon source > microbial density > pH. The maximum phosphate solubilizing capacity (616.81 mg·L-1) could be achieved under the following condition with 10.00 g·L-1 glucose, 0.79 g·L-1 urea, 35 ℃, the initial pH of medium 6.0, 3.8% microbial density and 160 r·min-1 rotating speed.