Biological effects of root exudates from resistant and susceptible banana varieties on Fusaiurm oxysporum f. sp. cubense and Bacillus subtilis

doi:10.13287/j.1001-9332.202007.039

Abstract

Abstract: Root exudates of banana resistant variety (‘Nantianhuang') and susceptible variety (‘Guijiao No. 6') to Fusarium wilt were collected in vitro by bathing root system to examine the biological effects of root exudates from banana varieties on Fusaiurm oxysporum f. sp. cubense and Bacillus subtilis. We explored the effects of root exudates of different banana varieties on the abundance of soil microorganisms and the growth of F. oxysporum f. sp. cubense and B. subtilis. The results showed that root exudates from resistant variety could significantly reduce the abundance of soil fungi and inhibit the spore germination of F. oxysporum f. sp. cubense. Root exudates from susceptible variety could significantly stimulate mycelia growth and spores germination, whereas root exudates from the tested banana varieties could significantly increase the growth and biofilm formation of B. subtilis. By dealing with the root exudates of resistant and susceptible varieties, the growth rate of mycelia were 11.28 and 12.28 mm·d^-1, and the germination rate of spores were 34.6% and 79.5%, respectively. After culturing for 12 h, the growth rates of B. subtilis (OD₆₀₀) were 1.27 and 1.14, and the biofilm formation (OD₅₇₀) were 1.11 and 1.30 after static culturing 72 h, respectively. There were significant differences between the values of resistant and susceptible varieties. The colonization amount of B. subtilis in the rhizosphere of susceptible variety was significantly higher than that of resistant variety. The contents of free amino acids and organic acid in root exudates of the resistant variety were higher than that of susceptible variety. The content ratio of acetic acid and proline in the root exudates of resistant variety were 3.7 times and 2.4 times of that of susceptible variety. In conclusion, root exudates of banana resistant variety could inhibit the growth of F. oxysporum f. sp. cubense. Root exudates from susceptible variety could promote the growth of F. oxysporum f. sp. cubense,while that from the tested banana varieties could all significantly enhance growth, biofilm formation and colonization ability of B. subtilis.

Key words: banana, root exudates, Fusaiurm oxysporum f. sp. cubense, Bacillus subtilis, biological effect

GAN Lin, DAI Yu-li, YANG Xiu-juan, DU Yi-xin, SHI Niu-niu, RUAN Hong-chun, CHEN Fu-ru. Biological effects of root exudates from resistant and susceptible banana varieties on Fusaiurm oxysporum f. sp. cubense and Bacillus subtilis[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2279-2286.

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