Effect of cropping patterns onbacterial community diversity and metabolic function in rhizosphere soil of Angelica sinensis

doi:10.13287/j.1001-9332.202112.031

Abstract

Abstract: The sustainable development of Angelica sinensis industry is seriously restricted by continuous cropping obstacles. In order to explore an efficient cultivation technique for A. sinensis, an experiment with five cropping patterns [A: Pisum sativum (Ps)-A. sinensis (As)-As, control); B: Ps-Triticum aestivum (Ta)-As; C: Ps-Mongolia astragalus (Ma)-As; D: Ps-Solanum tuberosum (St)-As); E: Ps-Fallow (F)-As)] were conducted in major A. sinensis producing areas located in Weiyuan County, Gansu Province. The physicochemical properties and relative abundance of bacterial genomic DNA in rhizosphere soil under different cropping patterns were measured during A. sinensis harvest period to investigate the effects of different cropping patterns on physicochemical properties, bacterial community diversity, and metabolic pathways. The results showed that: 1) the physicochemical properties in A. sinensis rhizosphere soil varied among different cropping patterns. Compared with the control, soil electrical conductivity under C pattern was significantly higher, and lower under B, D and E, CO₂ respiration rate for B, C, D and E were significantly increased. 2) Soil bacteria of A. sinensis rhizosphere soil in the five cropping patterns belonged to 26 phyla and 368 genera. The dominant genera were Gemmatimonas from Gemmatimonadetes, Sphingomonas from Proteobacteria, and Subgroup_6 from Acidobacteria. Compared with the control, the relative abundance of Proteobacteria and Actinobacteria under B and C patterns was significantly higher, Acidobacteria in D pattern was significantly lower, while Proteobacteria, Acidobacteria, and Actinobacteria in E pattern was significantly higher. 3) There were significantly negative relations between soil pH, electrical conductivity, contents of organic matter, available nitrogen, phosphorus and potassium with the relative abundance of Proteobacteria in A. sinensis rhizosphere soil across the five cropping patterns. 4) There was significant difference in relative abundance for bacteria of six metabolic pathways under the five cropping patterns. In conclusion, C pattern had a regulating effect on physicochemical properties and bacterial communities in A.sinensis rhizosphere soil, which could be taken as a major practice to overcome the continuous cropping obstacles.

Key words: cropping patterns, Angelica sinensis, rhizosphere soil, physicochemical properties, bacteria communities, metabolic pathways

JIANG Xiao-feng, GUO Feng-xia, CHEN Yuan, GUO Jian-guo, LIU Xiao-feng. Effect of cropping patterns onbacterial community diversity and metabolic function in rhizosphere soil of Angelica sinensis[J]. Chinese Journal of Applied Ecology, 2021, 32(12): 4254-4262.

References

[1] 康天兰, 刘学周, 曹占凤, 等. 甘肃中药材生产形势分析及发展对策. 甘肃农业科技, 2019(1): 90-94 [Kang T-L, Liu X-Z, Cao Z-F, et al. Situation analysis and development countermeasures of traditional Chinese medicine production in Gansu province. Gunsu Agricultural Science and Technology, 2019(1): 90-94]
[2] 张新慧, 张恩和, 郎多勇, 等. 不同茬口对当归根际土壤酶活性及其产量和品质的影响. 中草药, 2011, 42(11): 2322-2325 [Zhang X-H, Zhang E-H, Lang D-Y, et al. Effect of different cropping rotations on enzyme activities in rhizosphere soil and production quality of Angelica sinensis. Chinese Traditional and Herbal Drugs, 2011, 42(11): 2322-2325]
[3] 叶文斌, 樊亮. 当归轮作和连作根际土壤中致病菌变化研究. 湖北农业科学, 2014, 53(18): 4364-4367, 4392 [Ye W-B, Fan L. Changes of pathogenic bacteria numbers in rhizosphere of continuous cropping and rotation of Angelica sinensis. Hubei Agricultural Sciences, 2014, 53(18): 4364-4367, 4392]
[4] 韩剑, 张静文, 徐文修, 等. 新疆连作、轮作棉田可培养的土壤微生物区系及活性分析. 棉花学报, 2011, 23(1): 69-74 [Han J, Zhang J-W, Xu W-X, et al. Dynamics analysis of culturable soil microflora and microbial activity in continuous and rotation cropping systems of Xinjiang. Cotton Science, 2011, 23(1): 69-74]
[5] 王惠珍, 张新慧, 李应东, 等. 轮作与连作当归光合特性和挥发油的比较. 草业学报, 2011, 20(1): 69-74 [Wang H-Z, Zhang X-H, Li Y-D, et al. Comparison of photosynthetic characteristic and the essential oils in crop rotation and continuous cropping of Angelica sinensis. Acta Prataculturae Sinica, 2011, 20(1): 69-74]
[6] 韦翡翡, 吕蓉, 何微微, 等. 基于遥感技术进行渭源县当归种植分布信息提取研究. 中国中药杂志, 2019, 44(19): 4125-4128 [Wei F-F, Lyu R, He W-W, et al. Extraction of distribution information of Angelicae sinensis plants in Weiyuan County based on remote sensing technology. China Journal of Chinese Materia Medica, 2019, 44(19): 4125-4128]
[7] 巩杰, 陈利顶, 傅伯杰, 等. 黄土丘陵区小流域土地利用和植被恢复对土壤质量的影响. 应用生态学报, 2004, 15(12): 2292-2296 [Gong J, Chen L-D, Fu B-J, et al. Effects of land use and vegetation restoration on soil quality in a small catchment of the Loess Plateau. Chinese Journal of Applied Ecology, 2004, 15(12): 2292-2296]
[8] 贾松伟, 贺秀斌, 陈云明. 黄土丘陵区退耕撂荒对土壤有机碳的积累及其活性的影响. 水土保持学报, 2004, 18(3): 78-84 [Jia S-W, He X-B, Chen Y-M. Effect of land abandonment on soil organic carbon sequestration in loess hilly areas. Journal of Soil and Water Conservation, 2004, 18(3): 78-84]
[9] 巩杰, 陈利顶, 傅伯杰, 等. 黄土丘陵区小流域植被恢复的土壤养分效应研究. 水土保持学报, 2005, 19(1): 93-96 [Gong J, Chen L-D, Fu B-J, et al. Effects of vegetation restoration on soil nutrient in a small catchment in hilly loess area. Journal of Soil and Water Conservation, 2005, 19(1): 93-96]
[10] 时鹏, 高强, 王淑平, 等. 玉米连作及其施肥对土壤微生物群落功能多样性的影响. 生态学报, 2010, 30(22): 6173-6182 [Shi P, Gao Q, Wang S-P, et al. Effects of continuous cropping of corn and fertilization on soil microbial community functional diversity. Acta Ecologica Sinica, 2010, 30(22): 6173-6182]
[11] 于永强, 宇万太, 张璐. 海伦撂荒地植物生物量的季节变化. 应用生态学报, 2002, 13(6): 685-688 [Yu Y-Q, Yu W-T, Zhang L. Season fluctuation of plant biomass in land remained bare in Hailun experimental station. Chinese Journal of Applied Ecology, 2002, 13(6): 685-688]
[12] 马望力, 南志标. 拉萨地区不同春箭筈豌豆轮作模式的生产力评价. 草业科学, 2019, 36(6): 1616-1623 [Ma W-L, Nan Z-B. Evaluation of productivity of diffe-rent Vicia sativa cropping systems in the Lhasa area of Tibet, China. Pratacultural Science, 2019, 36(6): 1616-1623]
[13] 金彦博, 郭凤霞, 陈垣, 等. 岷县不同茬口对当归苗栽生长及抗病性的影响. 草业学报, 2018, 27(4): 69-78 [Jin Y-B, Guo F-X, Chen Y, et al. Effect of various crop residues on growth and disease resistance of Angelica sinensis seedings in Min County. Acta Prataculturae Sinica, 2018, 27(4): 69-78]
[14] 王田涛, 王琦, 王惠珍, 等. 连作条件下间作模式对当归生长特性和产量的影响. 草业学报, 2013, 22(2): 54-61 [Wang T-T, Wang Q, Wang H-Z, et al. Effects of intercropping patterns on growth characters and yield of Angelica sinensis under continuous mono-cropping planting. Acta Prataculturae Sinica, 2013, 22(2): 54-61]
[15] 李林强, 邱黛玉, 贾雪. 连作轮作模式下当归大蒜间作对当归质量的影响. 干旱地区农业研究, 2017, 35(3): 53-58 [Li L-Q, Qiu D-Y, Jia X. Effects of continuous cropping and rotation planting patterns on the quality of angelica under intercropping of angelica and garlic. Agricultural Research in the Arid Areas, 2017, 35(3): 53-58]
[16] 霍庆迪, 赵庆芳, 马艳, 等. 不同耕作方式对当归根际土壤细菌群落多样性的影响. 广西植物, 2018, 38(2): 241-249 [Huo Q-D, Zhao Q-F, Ma Y, et al. Effects of different cultivation methods on bacterial community diversity in rhizosphere soil of Angelica sinensis. Guihaia, 2018, 38(2): 241-249]
[17] 王田涛, 王琦, 王惠珍, 等. 当归自毒作用和其对不同作物的化感效应. 草地学报, 2012, 20(6): 1132-1138 [Wang T-T, Wang Q, Wang H-Z, et al. Autotoxi-city of Angelica sinensis and allelopathy on tested plants. Acta Agrestia Sinica, 2012, 20(6): 1132-1138]
[18] 张新慧, 张恩和, 王永捷, 等. 不同前茬对当归药材产量及品质的影响. 中药材, 2010, 33(11): 1678-1680 [Zhang X-H, Zhang E-H, Wang Y-J, et al. Effects of different previous crop on yield and quality of Angelica sinensis. Joumal of Chinese Medicinal Mate-rials, 2010, 33(11): 1678-1680]
[19] 袁洪超, 郭凤霞, 陈垣, 等. 高寒区轮作模式对当归田土壤特性及药材产量的影响. 草业学报, 2018, 27(10): 183-193 [Yuan H-C, Guo F-X, Chen Y, et al. Effect of crop roprotation patterns on field soil properties and medicinal material yield for Angelicae sinensis in alpineregions. Acta Prataculturae Sinica, 2018, 27(10): 183-193]
[20] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2011: 495 [Bao S-D. Soil and Agrochemistry Analysis. Beijing: China Agriculture Press, 2011: 495]
[21] Xu H, Chen ZY, Wu XY, et al. Antibiotic contamination amplifies the impact of foreign antibiotic-resistant bacteria on soil bacterial community. Science of the Total Environment, 2021, 758: 143693-143701
[22] Magoc T, Salzberg SL. FLASH: Fast length adjustment of short reads to improve genome assemblies. Bioinformatics, 2011, 27: 2957-2963
[23] Edgar RC. UPARSE: Highly accurate OUT sequences from microbial amplicon reads. Nature Methods, 2013, 10: 966-998
[24] Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing date. Nature Methods, 2010, 7: 335-336
[25] 彭政, 郭秀芝, 徐扬, 等. 药用植物与根际微生物互作的研究进展与展望. 中国中药杂志, 2020, 45(9): 2023-2030 [Peng Z, Guo X-Z, Xu Y, et al. Advances in interaction between medicinal plants and rhizosphere microoganisms. China Journal of Chinese Materia, 2020, 45(9): 2023-2030]
[26] 姜小凤, 郭凤霞, 陈垣, 等. 休耕和种植作物对黑麻土壤肥力的影响. 水土保持学报, 2021, 35(1): 229-235 [Jiang X-F, Guo F-X, Chen Y, et al. Effect of fallowing and planting crops on soil fertility of black flax soil. Journal of Soil and Water Conservation, 2021, 35(1): 229-235]
[27] 杨美玲, 张霞, 王绍明, 等. 基于高通量测序的裕民红花根际土壤细菌群落特征分析. 微生物学通报, 2018, 45(11): 2429-2438 [Yang M-L, Zhang X, Wang S-M, et al. High throughput sequencing analysis of bacterial communities in Yumin safflower. Microbiology China, 2018, 45(11): 2429-2439]
[28] Lauber CL, Hamady M, Knight R, et al. Pyrosequen-cing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale. Applied and Environmental Microbiology, 2009, 75(15): 5111-5120
[29] 杨彦明, 周祎, 张博文, 等. 连续深松对黑土区玉米根际土壤细菌群落结构的影响. 土壤与作物, 2020(4): 367-379 [Yang Y-M, Zhou W, Zhang B-W, et al. Effects of continuous subsoiling on bacterial community structure in rhizosphere soil of maize in Mollisols. Soils and Crops, 2020(4): 367-379]
[30] 李锐, 刘瑜, 褚贵新. 不同种植方式对绿洲农田土壤酶活性与微生物多样性的影响. 应用生态学报, 2015, 26(2): 490-496 [Li R, Liu Y, Chu G-X. Effects of different cropping patterns on soil enzyme activities and soil microbial community diversity in oasis farmland. Chinese Journal of Applied Ecology, 2015, 26(2): 490-496]
[31] Clarke KR. Non-parametric multivariate analysis of changes in community structure. Australian Journal of Ecology, 1993, 18: 117-143
[32] 袁红朝, 吴昊, 葛体达, 等. 长期施肥对稻田土壤细菌、古菌多样性和群落结构的影响. 应用生态学报, 2015, 26(6): 1807-1813 [Yuan H-C, Wu H, Ge T-D, et al. Effects of long-term fertilization on bacterial and archaea diversity and community structure in paddy soil. Chinese Journal of Applied Ecology, 2015, 26(6): 1807-1813]
[33] 周柳婷, 李建鹃, 赵艳琳, 等. 基于高通量测序的连栽木麻黄根际土壤细菌群落变化研究. 生态学报, 2020, 40(8): 2670-2679 [Zhou L-T, Li J-J, Zhao Y-L, et al. Variation of bacterial communities in the rhizosphere soils of successive rotations Casuarina equisetifolia plantations based on high-throughput sequencing analysis. Acta Ecologica Sinica, 2020, 40(8): 2670-2679]
[34] Sousa RMS, Mendes LW, Antunes JEL, et al. Diversity and structure of bacterial community in rhizosphere of lima bean. Applied Soil Ecology, 2019, 150: 103490, https://doi.org/10.1016/j.apsoil.2019.103490
[35] 南丽丽, 郭全恩, 刘雪强, 等. 荒漠灌区首蓓后茬轮作不同作物对土壤细菌群落的影响. 草地学报, 2021, 29(1): 25-32 [Nan L-L, Guo Q-E , Liu X-Q, et al. Effects of different crop rotation on soil bacterial community in desert irrigated area. Acta Agrestia Sinica, 2021, 29(1): 25-32]
[36] 尹国丽, 李亚娟, 张振粉, 等. 不同草田轮作模式土壤养分及细菌群落组成特征. 生态学报, 2020, 40(5): 1542-1550 [Yin G-L, Li Y-J, Zhang Z-F, et al. Characteristics of soil nutrients and bacterial community composition under different rotation patterns in grassland. Acta Ecologica Sinica, 2020, 40(5): 1542-1550]
[37] Grice EA, Kong HH, Conlan S, et al. Topographical and temporal diversity of the human skin microbiome. Science, 2009, 324: 1190-1192
[38] 厉桂香, 马克明. 北京东灵山树线处土壤细菌的PICRGSt基因预测分析. 生态学报, 2018, 38(6): 2180-2186 [ Li G-X, Ma K-M. PICRUSt-based predicted metagenomic analysis of treeline soil bacteria on Mount Dongling, Beijing. Acta Ecologica Sinica, 2018, 38(6): 2180-2186]
[39] 张睿洋. 放牧对短花针茅荒漠草原生物多样性和生态系统功能的影响. 博士论文. 呼和浩特: 内蒙古农业大学, 2018 [Zhang R-Y. Effects of grazing on biodiversity and ecosystem of Stipa breviflora desert steppe. PhD Thesis. Huhhot: Inner Mongolia Agricultural University, 2018]
[40] 杜宇佳, 高广磊, 陈丽华, 等. 呼伦贝尔沙区土壤细菌群落结构与功能预测. 中国环境科学, 2019, 39(11): 4840-4848 [Du Y-J, Gao G-L, Chen L-H, et al. Prediction of soil bacterial community structure and function in Hulum Buir sand area. China Environmental Sciences, 2019, 39(11): 4840-4848]
[41] 杨盼, 翟亚萍, 赵祥, 等. 丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析. 微生物学通报, 2020, 47(11): 3868-3879 [Yang P, Zhai Y-P, Zhao X, et al. Effect of interaction between arbuscular mycorrhizal fungi and Rhizobium on Medicago sativa rhizospheresoil bacterial community structure and PICRUSt functional prediction. Microbioligy China, 2020, 47(11): 3868-3879]