Effects of different fertilization patterns on soil enzyme activities in greenhouse vegetable field

doi:10.13287/j.1001-9332.201603.015

Abstract

Abstract: A fixed-site greenhouse vegetable fertilization experiment was carried out to study effects of 6 fertilization patterns on soil enzyme activities in Tianjin City, Northern China. The results showed that during the growing stages of tomato, activities of soil α-glucosidase, β-xylosidase, β-glucosidase, β-cellobiosidase, chitinase and phosphatase in different treatments all increased first and then decreased, while soil urease activities increased first and then became flat. Compared with the chemical nitrogen fertilizer treatment, soil enzyme activities were much higher in treatments of combined application of organic materials with chemical fertilizers, and rose with the increasing input of pig manure and especially the application of straw. A significant positive correlation was found between soil enzyme activities, microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) contents at different growing stages of tomato. Under the condition of same nutrient input, the combined application of inorganic fertilizers with organic materials, especially a certain amount of corn straw, was capable of increasing soil enzyme activities and keeping soil fertility and sustainability in greenhouse vegetable production.

WANG Wen-feng, LI Chun-hua, HUANG Shao-wen, GAO Wei, TANG Ji-wei. Effects of different fertilization patterns on soil enzyme activities in greenhouse vegetable field[J]. Chinese Journal of Applied Ecology, 2016, 27(3): 873-882.

References

[1] Li T-L (李天来). Current situation and prospects of greenhouse industry development in China. Journal of Shenyang Agricultural University (沈阳农业大学学报), 2005, 36(2): 131-138 (in Chinese)
[2] Zhang T-L (张桃林), Li Z-P (李忠佩), Wang X-X (王兴祥). Soil degradation and its eco-environmental impact under highly-intensified agriculture. Acta Pe-dologica Sinica (土壤学报), 2006, 43(5): 843-850 (in Chinese)
[3] Chen Z-J (陈竹君), Gao J-J (高佳佳), Zhao W-Y (赵文艳), et al. Effects of application of phosphorus and potassium fertilizers on ion compositions of soil solution in solar greenhouse. Transactions of the Chinese Society of Agricultural Engineering (农业工程学报), 2011, 27(2): 261-266 (in Chinese)
[4] Huang S-W (黄绍文), Wang Y-J (王玉军), Jin J-Y (金继运), et al. Status of salinity, pH and nutrients in soils in main vegetable production regions in China. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 2011, 17(4): 906-918 (in Chinese)
[5] Li T-X (李廷轩), Zhou J-M (周健民), Duan Z-Q (段增强), et al. Nutrient management of greenhouse cropping systems in China. Journal of Soil and Water Conservation (水土保持学报), 2006, 19(4): 70-75 (in Chinese)
[6] Hu YC, Song ZW, Lu WL, et al. Current soil nutrient status of intensively managed greenhouses. Pedosphere, 2012, 22: 825-833
[7] Bandick AK, Dick RP. Field management effects on soil enzyme activities. Soil Biology and Biochemistry, 1999, 31: 1471-1479
[8] Badiane NNY, Chotte JL, Pate E, et al. Use of soil enzyme activities to monitor soil quality in natural and improved fallows in semi-arid tropical regions. Applied Soil Ecology, 2001, 18: 229-238
[9] DeForest JL. The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and L-DOPA. Soil Biology and Biochemistry, 2009, 41: 1180-1186
[10] Tian Y-Q (田永强), Cao Z-F (曹之富), Zhang X-Y (张雪艳), et al. Changes of soil enzyme activities under different agricultural treatments in greenhouse and its correlation analysis. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 2009, 15(4): 857-864 (in Chinese)
[11] Aon MA, Colaneri AC. Temporal and spatial evolution of enzymatic activities and physico-chemical properties in an agricultural soil. Applied Soil Ecology, 2001, 18: 255-270
[12] Yang W-Q (杨万勤), Wang K-Y (王开运). Advances on soil enzymology. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2002, 8(5): 564-570 (in Chinese)
[13] Liu S-J (刘善江), Xia X (夏雪), Chen G-M (陈桂梅), et al. Study progress on functions and affecting factors of soil enzymes. Chinese Agricultural Science Bulletin (中国农学通报), 2011, 27(21): 1-7 (in Chinese)
[14] Wu F-Z (吴凤芝), Meng L-J (孟立君), Wang X-Z (王学征). Soil enzyme activities in vegetable rotation and continuous cropping system under shed protection. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 2006, 12(4): 554-558 (in Chinese)
[15] Sun C-J (孙彩菊), Cheng Z-H (程智慧), Meng H-W (孟焕文), et al. Variation of soil microorganism quantities and enzyme activities at the third year cultivation of continuous positional intercropping of tomato with garlic under plastic tunnel. Journal of Northwest A&F University (Natural Science) (西北农林科技大学学报:自然科学版), 2012, 40(12): 97-105 (in Chinese)
[16] Sun Y-W (孙艺文), Wu F-Z (吴凤芝). Effects of wheat and oat residues on growth of continuous cucumber cropping and soil enzymatic activity. China Vegetables (中国蔬菜), 2013(4): 46-51 (in Chinese)
[17] Zhang H (张浩), Zhao J-Z (赵九洲), Zhang L (张丽), et al. Effects of different proportion of soybean stubble and continuous cropping soil on tomato growth and development and rhizosphere soil environment. Acta Agriculturae Boreali-Occidentalis Sinica (西北农业学报), 2014, 23(7): 138-145 (in Chinese)
[18] Ma Y-H (马云华), Wei M (魏珉), Wang X-F (王秀峰). Variation of microflora and enzyme activity in continuous cropping cucumber soil in solar greenhouse. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(6): 1005-1008 (in Chinese)
[19] Wu Z-H (吴忠红), Du X-M (杜新民), Zhang Y-Q (张永清), et al. The microbe amount and enzymes activities of soil in greenhouse in Jinnan area. Chinese Agricultural Science Bulletin (中国农学通报), 2007, 23(1): 296-298 (in Chinese)
[20] Zhao Q (赵秋), Gao X-B (高贤彪), Ning X-G (宁晓光), et al. Changes of pH and enzyme activities in greenhouse soils of different planting years in Tianjin. Acta Agriculturae Boreali-Sinica (华北农学报), 2012, 27(1): 215-217 (in Chinese)
[21] Caldwell BA. Enzyme activities as a component of soil biodiversity: A review. Pedobiologia, 2005, 49: 637-644
[22] Marx MC, Wood M, Jarvis SC. A microplate fluorime-tric assay for the study of enzyme diversity in soils. Soil Biology and Biochemistry, 2001, 33: 1633-1640
[23] Stemmer M. Multiple-substrate enzyme assays: A useful approach for profiling enzyme activity in soils? Soil Biology and Biochemistry, 2004, 36: 519-527
[24] Lin X-G (林先贵). Principles and Methods of Soil Microbiology Research. Beijing: Higher Education Press, 2010 (in Chinese)
[25] Wu J-S (吴金水), Lin Q-M (林启美), Huang Q-Y (黄巧云), et al. Soil Microbial Biomass Methods and Application. Beijing: China Meteorological Press, 2006 (in Chinese)
[26] Zhong Z, Makeschin F. Soluble organic nitrogen in temperate forest soils. Soil Biology and Biochemistry, 2003, 35: 333-338
[27] Pan G, Zhou P, Li Z, et al. Combined inorganic/orga-nic fertilization enhances N efficiency and increases rice productivity through organic carbon accumulation in a rice paddy from the Tai Lake region, China. Agriculture, Ecosystems & Environment, 2009, 131: 274-280
[28] Tong C, Xiao H, Tang G, et al. Long-term fertilizer effects on organic carbon and total nitrogen and coupling relationships of C and N in paddy soils in subtropical China. Soil and Tillage Research, 2009, 106: 8-14
[29] Li Z, Liu M, Wu X, et al. Effects of long-term chemical fertilization and organic amendments on dynamics of soil organic C and total N in paddy soil derived from barren land in subtropical China. Soil and Tillage Research, 2010, 106: 268-274
[30] Wan Z-M (万忠梅), Song C-C (宋长春). Advance on response of soil enzyme activity to ecological environment. Chinese Journal of Soil Science (土壤通报), 2009, 40(4): 951-956 (in Chinese)
[31] Zhang Y-M (张咏梅), Zhou G-Y (周国逸), Wu N (吴宁). A review of studies on soil enzymology. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 2004, 12(1): 83-90 (in Chinese)
[32] Wan Z-M (万忠梅), Wu J-G (吴景贵). Study progress on factors affecting soil enzyme activity. Journal of Northwest A&F University (Natural Science) (西北农林科技大学学报:自然科学版), 2005, 33(6): 87-92 (in Chinese)
[33] Kanchikerimath M, Singh D. Soil organic matter and biological properties after 26 years of maize-wheat-cowpea cropping as affected by manure and fertilization in a Cambisol in semiarid region of India. Agriculture, Ecosystems & Environment, 2001, 86: 155-162
[34] Bastida F, Kandeler E, Hernández T, et al. Long-term effect of municipal solid waste amendment on microbial abundance and humus-associated enzyme activities under semiarid conditions. Microbial Ecology, 2008, 55: 651-661
[35] Gu Y, Zhang X, Tu S, et al. Soil microbial biomass, crop yields, and bacterial community structure as affec-ted by long-term fertilizer treatments under wheat-rice cropping. European Journal of Soil Biology, 2009, 45: 239-246
[36] Zhong W, Gu T, Wang W, et al. The effects of mineral fertilizer and organic manure on soil microbial community and diversity. Plant and Soil, 2010, 326: 511-522
[37] Olander LP, Vitousek PM. Regulation of soil phosphatase and chitinase activity by N and P availability. Biogeochemistry, 2000, 49: 175-191
[38] Zhang B-Y (张北赢), Chen T-L (陈天林), Wang B (王兵). Effects of long-term uses of chemical ferti-lizers on soil quality. Chinese Agricultural Science Bulletin (中国农学通报), 2010, 26(11): 182-187 (in Chinese)
[39] Marx MC, Kandeler E, Wood M, et al. Exploring the enzymatic landscape: Distribution and kinetics of hydrolytic enzymes in soil particle-size fractions. Soil Bio-logy and Biochemistry, 2005, 37: 35-48
[40] Allison SD, Jastrow JD. Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biology and Biochemistry, 2006, 38: 3245-3256
[41] Wichern F, Mayer J, Joergensen RG, et al. Release of C and N from roots of peas and oats and their availability to soil microorganisms. Soil Biology and Biochemistry, 2007, 39: 2829-2839
[42] Garcla-Gil JC, Plaza C, Soler-Rovira P, et al. Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass. Soil Biology and Biochemistry, 2000, 32: 1907-1913
[43] Wu X-C (吴秀臣), Sun H (孙辉), Yang W-Q (杨万勤). Effects of elevated temperature and atmospheric carbon dioxide on activity of soil enzymes. Soils (土壤), 2007, 39(3): 358-363 (in Chinese)
[44] Joergensen RG, Brookes PC, Jenkinson DS. Survival of the microbial biomass at elevated temperatures. Soil Biology and Biochemistry, 1990, 22: 1129-1136
[45] Verburg PSJ, Dam VD, Hefting M, et al. Microbial transformation of C and N in a boreal forest floor as affected by temperature. Plant and Soil, 1999, 208: 187-197
[46] Aon MA, Sarena DE, Burgos JL, et al. (Micro) biolo-gical, chemical and physical properties of soils subjected to conventional or no-till management: An assessment of their quality status. Soil and Tillage Research, 2001, 60: 173-186
[47] Cao H (曹慧), Sun H (孙辉), Yang H (杨浩), et al. A review: Soil enzyme activity and its indication for soil quality. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2003, 9(1): 105-109 (in Chinese)
[48] Guo J-X (郭继勋), Lin H-J (林海俊). Enzymic acti-vity of alkaline meadow soil with different grassland ve-getations. Chinese Journal of Applied Ecology (应用生态学报), 1997, 8(4): 412-416 (in Chinese)
[49] Taylor JP, Wilson B, Mills MS, et al. Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biology and Biochemistry, 2002, 34: 387-401
[50] Fernandes SAP, Bettiol W, Cerri CC. Effect of sewage sludge on microbial biomass, basal respiration, metabo-lic quotient and soil enzymatic activity. Applied Soil Eco-logy, 2005, 30: 65-77
[51] Jiao X-G (焦晓光), Wei D (魏丹), Sui Y-Y (隋跃宇). Effects of long-term fertilization on the soil enzyme activities and soil nutrients of the black and dark brown soils. Chinese Journal of Soil Science (土壤通报), 2011, 42(3): 698-703 (in Chinese)