Effects of micro-fertilizers foliar spray on the content of main effective components of Angelica dahurica

doi:10.13287/j.1001-9332.201711.001

Abstract

Abstract: The zinc, boron and molybdenum were sprayed on the foliage of Angelica dahurica du-ring the vigorous growth period to explore the effects of the combination of zinc, boron and molybdenum fertilizers on the content of main effective components of A. dahurica, and to find out the optimum spraying amount. The results showed that the application of zinc, boron and molybdenum foliar spray was beneficial to increase the content of imperatorin and total cumarin, whereas had no effect on the content of the isoimperatorin. The effect of boron spray on the accumulation of imperatorin and total coumarin was the most significant, followed by the zinc and molybdenum. The interaction between boron and molybdenum had a negative cooperative effect on the content of imperatorin, while the interaction between zinc and molybdenum had a positivecooperative effect on the content of total coumarin. The contents of total coumarin reached over 0.7% respectively, with the complex fertilizer spray of zinc at 0.15-0.24 kg·hm^-2, boron at 2.02-2.36 kg·hm^-2 and molybdenum at 0.08-0.13 kg·hm^-2. The contents of imperatorin reached over 0.2% respectively, with the complex fertilizer spray of zinc at 0.15-0.20 kg·hm^-2, boron at 1.37-1.47 kg·hm^-2 and molybdenum at 0.09-0.13 kg·hm^-2. It was concluded that the application of zinc, boron and molybdenum foliar spray was beneficial to promote the quality of A. dahurica by enhancing its accumulation of coumarin.

Key words: trace element, imperatorin, Angelica dahurica, isoimperatorin, total coumarin

ZHANG Ya-qin, YANG Zheng-ming, SHI Feng, DOU Ming-ming, MA Liu-hui, CHEN Yu, LEI Fei-yi, CHEN Xing-fu. Effects of micro-fertilizers foliar spray on the content of main effective components of Angelica dahurica[J]. Chinese Journal of Applied Ecology, 2017, 28(11): 3505-3514.

References

[1] Editorial Committee of Flora of China (中国植物志编辑委员会). Flora Reipublicae Popularis Sinicae. Beijing: Science Press, 1998 (in Chinese)
[2] Ma Y-Y (马逾英), Guo D-D (郭丁丁), Jiang G-H (蒋桂华), et al. Investigation report on the germplasm resources of Angelica dahurica. West China Journal of Pharmaceutical Sciences (华西药学杂志), 2009, 24(5): 457-460 (in Chinese)
[3] Lechner D, Stavri M, Oluwatuyi M, et al. The anti-staphylococcal activity of Angelica dahurica (Bai Zhi). Phytochemistry, 2004, 65: 331-335
[4] Pervin M, Hasnat MD, Debnath T, et al. Antioxidant, anti-inflammatory and antiproliferative activity of Angelica dahurica root extracts. Journal of Food Biochemistry, 2014, 38: 281-292
[5] Lu X, Yuan ZY, Yan XJ, et al. Effects of Angelica dahurica on obesity and fatty liver in mice. Chinese Journal of Natural Medicines, 2016, 14: 641-652
[6] Zheng YM, Shen JZ, Wang Y, et al. Antioxidant and anti-cancer activities of Angelica dahurica extract via induction of apoptosis in colon cancer cells. Phytomedicine, 2016, 23: 1267-1274
[7] Wu K-Y (武孔云), Sun C (孙超). Quality of traditional Chinese medicinal materials and their promotion pathway. Chinese Traditional and Herbal Drugs (中草药), 2010, 41(7): 1210-1215 (in Chinese)
[8] Meng J (孟杰), Chen X-F (陈兴福), Yang W-Y (杨文钰), et al. Effect of combined application of zinc, boron and molybdenum on yield and saikosaponin a, saikosaponin d contents of Bupleurum chinense. China Journal of Chinese Materia Medica (中国中药杂志), 2014, 39(22): 4297-4303 (in Chinese)
[9] Han J-P (韩建萍), Liang Z-S (梁宗锁), Zhang W-S (张文生). Effect of microelement on growth and active ingredient of Salvia miltiorrhiza Bge. Journal of Plant Nutrition and Fertilizer (植物营养与肥料学报), 2005, 11(4): 560-563 (in Chinese)
[10] He Y-J (何跃君), Xue L (薛立). Biological effects of rare earth elements and their action mechanisms. Chinese Journal of Applied Ecology (应用生态学报), 2005, 16(10): 1983-1989 (in Chinese)
[11] Liang X-H (梁新华), Zhang F-X (张风侠), Wang J (王俊). Effects of molybdenum, boron, manganese and zinc on quality of cultured Glycyrrhiza uralensis Fisch. Journal of Plant Nutrition and Fertilizer (植物营养与肥料学报), 2011, 17(6): 1487-1494 (in Chinese)
[12] Tang L (汤璐), Lin J-H (林江辉), Yan G-X (闫广轩), et al. Effects of Cu, Zn and Se on contents of total flavonlid, chlorogenic acid and Se in the flower of Chrysanthemum morifolium. Journal of Plant Nutrition and Fertilizer (植物营养与肥料学报), 2009, 15(6): 1475-1480 (in Chinese)
[13] Xu J-Z (徐建中), Sheng S-J (盛束军), Yao J-F (姚金富). Regulation on growth and accumulation of total alkaloid in Leonurus artemisia (Lour.) S.Y.Hu by va-rious micro-fertilizers. China Journal of Chinese Materia Medica (中国中药杂志), 2000, 25(1): 20-23 (in Chinese)
[14] Xu J-Z (徐继振), Liu X-R (刘效瑞), Qi F-P (祁风鹏), et al. Application effect of Mo, Zn, Mn and B in cultivation of Angelica sinensis. China Journal of Chinese Materia Medica (中国中药杂志), 1998, 23(2): 81-83 (in Chinese)
[15] Chen J-W (陈郡雯), Wu W (吴卫), Hou K (侯凯), et al. Effects of fertilization ratios of N, P, and K on yield and active ingredients of Angelica dahurica var. formosana from Sichuan Province. Chinese Traditional and Herbal Drugs (中草药), 2011, 42(1): 153-157 (in Chinese)
[16] Yang F-R (杨富荣), Yang H-Y (杨海莹), Guo D-Z (郭敦志). A preliminary study on the methods of early bolting and its prevention of Angelica dahurica. Journal of Chinese Medicinal Materials (中药材), 2001, 24(10): 708 (in Chinese)
[17] Hou K (侯凯), Chen J-W (陈郡雯), Zhai J-Y (翟娟园), et al. Effect of different plant growth regulators on yield and quality of Angelica dahurica var. formosana development. China Journal of Chinese Materia Medica (中国中药杂志), 2013, 38(13): 2082-2085 (in Chinese)
[18] Chinese Phaimacopoeia Commission (中国药典委员会). Pharmacopoeia of the People’s Republic of China. Beijing: China Medical Science Press, 2015 (in Chinese)
[19] Zhang G-J (张贵君), Luo R (罗容), Wang Y-J (王奕洁). Theory of effective components of traditional Chinese medicine and traditional Chinese medicine group. Journal of Chinese Medicinal Materials (中药材), 2007, 30(2): 125-126 (in Chinese)
[20] Han J-P (韩建萍), Liang Z-S (梁宗锁), Wang J-M (王敬民). The relationship between mineral element and the root growth and accumulation of effective ingredient in root of traditional herbs. Plant Physiology Communications (植物生理学报), 2003, 39(1): 78-82 (in Chinese)
[21] Chen J-W (陈郡雯), Wu W (吴卫), Hou K (侯凯), et al. Dynamic research of growth, N, P, K and active ingredients in Angelica dahurica var. formosana from Sichuan Province. China Journal of Chinese Materia Medica (中国中药杂志), 2010, 35(21): 2812-2817 (in Chinese)
[22] Cheng Y-A (成延鏊), Yang D-G (杨定国), Wen Y-M (温琰茂). Contents of boron and zinc in purplish soil and yellow earth of Sichuan and evaluation for agriculture. Scientia Geographica Sinica (地理科学), 1986, 6(2): 141-150 (in Chinese)
[23] Navarro LE, Barrameda MY, Lentini M, et al. Compa-rative study of Zn deficiency in L. sativa and B. oleracea plants: NH₄⁺ assimilation and nitrogen derived protective compounds. Plant Science, 2016, 248: 8-16
[24] Zhou S-M (周苏玫), Fan H (樊骅), Guo J-H (郭俊红), et al. Effects of organic manure and microelement fertilizer on the output and quality of peanut. Journal of Henan Agricultural University (河南农业大学学报), 2003, 37(4): 335-338 (in Chinese)
[25] Du Y-Q (杜应琼), Liao X-R (廖新荣), Huang Z-Y (黄志尧), et al. Effect of boron and molybdenum on nitrogen metabolism of peanut. Acta Agronomica Sinica (作物学报), 2001, 27(5): 612-616 (in Chinese)
[26] Li L (李路), Hu C-X (胡承孝), Tan Q-L (谭启玲), et al. Effects of Mo pollution on photosynthesis characteristics and yields of winter wheat. Journal of Agro-Environment Science (农业环境科学学报), 2016, 35(4): 620-626 (in Chinese)
[27] Zong Y-Z (宗毓铮), Wang W-Y (王雯玥), Han Q-F (韩清芳), et al. Effects of different levels of boron fertilizer on alfalfa photosynthesis and source-sink translocation of soluble carbohydrate in alfalfa. Acta Agronomica Sinica (作物学报), 2010, 36(4): 665-672 (in Chinese)
[28] Shi M-T (施木田), Chen R-K (陈如凯). Effects of zinc and boron nutrition on balsampear (Momordica charantia) yield and quality, and polyamines, hormone, and senescence of its leaves. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(1): 77-80 (in Chinese)
[29] Zhai J-Y (翟娟园), Wu W (吴卫), Liao K (廖凯), et al. Effects of soil factors on yield and quality of Angelica dahurica var. formosana. Chinese Traditional and Herbal Drugs (中草药), 2010, 41(6): 984-988 (in Chinese)
[30] Kovcs B, Pusks PA, Huzsvai L, et al. Effect of molybdenum treatment on molybdenum concentration and nitrate reduction in maize seedlings. Plant Physiology & Biochemistry, 2015, 96: 38-44