Comprehensive evaluation and construction of drought resistance index system in Hydrangea macrophylla

doi:10.13287/j.1001-9332.201810.002

Abstract

Abstract: With the continuous drought stress treatment to five Hydrangea macrophylla varieties of different abilities of drought resistance, twenty-five physiological-biochemical indices were mea-sured. We evaluated the drought resistance of different varieties and established the mathematic model. The results showed that leaf mass per area, cell membrane permeability (CMP), the content of malonaldehyde (MDA), the activity of superoxide dismutase (SOD), soluble sugar content, proline content, intercellular carbon dioxide and non-photochemical quenching coefficient were significantly increased under drought stress for twenty days. In contrast, relative water content, net photosynthetic rate, stomatal conductance, transpiration rate, actual photochemical efficiency of PSⅡ and electron transport rate (ETR) were significantly decreased. The principal component analysis classified those indices into three independent comprehensive indices (accumulative contribution of 87.1%). The principal component 1 mainly reflected the information of photosynthetic and fluorescence. The principal component 2 mainly reflected the information of plant vigor. The principal component 3 mainly reflected the information of membrane system and osmotic adjust system. Five H. macrophylla varieties were divided into three categories by clustering analysis: drought-tolerant type (including H. macrophylla ‘Lavblaa’) and H. macrophylla ‘Taube’), intermediate type (H. macrophylla ‘You and me romance’), and drought-intolerant type (including H. macrophylla cv. Endless summer bride and H. macrophylla ‘Tricolor’). Comprehensive evaluation of drought resis-tance (D value) showed that the ability of drought resistance decreased in order of H. macrophylla ‘Lavblaa’ > H. macrophylla ‘Taube’> H. macrophylla ‘You and me romance’> H. macrophylla cv. Endless summer bride > H. macrophylla ‘Tricolor’. Four influential indices for drought resis-tance including CMP, the activity of peroxidase (POD), soluble protein contents (SP) and ETR was screened by stepwise regression analysis, which could be used for the rapid identification of drought resistance of H. macrophylla.

CAI Jian-guo, ZHANG Yi, SUN Ou-wen, YANG Qian-qian. Comprehensive evaluation and construction of drought resistance index system in Hydrangea macrophylla[J]. Chinese Journal of Applied Ecology, 2018, 29(10): 3175-3182.

References

[1] Katarzyna J, Ilona CM, Izabela M. Polyamines in yellow lupin (Lupinus luteus L.) tolerance to soil drought. Acta Physiologiae Plantarum, 2017, 39: 202-212
[2] Fang YJ, Xiong LZ. General mechanisms of drought response and their application in drought resistance improvement in plants. Cellular & Molecular Life Sciences, 2015, 72: 673-689
[3] Zala K, Dominik V, Stanislav M, et al. Hop (Humulus lupulus L.) response mechanisms in drought stress: Proteomic analysis withphysiology. Plant Physiology and Biochemistry, 2016, 105: 67-78
[4] Wang L-L (王林龙), Li Q-H (李清河), Xu J (徐军), et al. Morphology and physiology characteristic responses of different provenances of Artemisia ordosica to drought stress. Scientia Silvae Sinicae (林业科学), 2015, 51(2): 37-43 (in Chinese)
[5] Antonino DI, Antonino M, Gabriella SS, et al. Fine root growth of Quercus pubescens seedlings after drought stress and fire disturbance. Environmental and Experimental Botany, 2011, 74: 272-279
[6] Zhang Z-M (张智猛), Wan S-B (万书波), Dai L-X (戴良香), et al. Estimating and screening of drought resistance indexes of peanut. Chinese Journal of Plant Ecology (植物生态学报), 2011, 35(1): 100-109 (in Chinese)
[7] Nesi B, Lazzereschi S, Pecchioli S, et al. Effect of colored shade netting on the vegetative development and on the photosynthetic activity in several Hydrangea genotypes. Acta Horticulturae, 2013, 1000: 345-352
[8] Yu X-M (虞秀明), Sun Q (孙强), Yao H-J (姚红军), et al. Comparative photosynthetic characteristics of different Hydrangea L. varieties. Plant Physiology Journal (植物生理学报), 2016, 52(8): 1142-1150 (in Chinese)
[9] Cai J-G (蔡建国), Wei M-Q (韦孟琪), Zhang Y (章毅), et al. Effects of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in lea-ves of Hydrangea macrophylla. Chinese Journal of Plant Ecology (植物生态学报), 2017, 41(5): 570-576 (in Chinese)
[10] Subrahmanyam D, Subash N, Haris A, et al. Influence of water stress on leaf photosynthetic characteristics in wheat cultivars differing in their susceptibility to drought. Photosynthetica, 2015, 44: 125-129
[11] Mo Y-L (莫言玲), Zheng J-X (郑俊鶱), Yang R-P (杨瑞平), et al. Physiological response and tolerance to drought stress of different watermelon genotypes. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(6): 1942-1952 (in Chinese)
[12] Mao Z, Jiang H, Wang Y, et al. Water balance of birch and larch leaves and their resistance to short and progressives soil drought. Russian Journal of Plant Physiology, 2004, 51: 697-701
[13] Li H-S (李合生). Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2006 (in Chinese)
[14] Srivastava S, Srivastava M. Morphological changes and antioxidant activity of Stevia rebaudiana under water stress. American Journal of Plant Sciences, 2014, 5: 3417-3422
[15] Chen H, Jiang JG. Osmotic adjustment and plant adaptation to environmental changes related to drought and salinity. Environmental Reviews, 2010, 18: 309-319
[16] Li G-H (厉广辉), Wan Y-S (万勇善), Liu F-Z (刘风珍), et al. Photosynthetic characteristics in different peanut cultivars under conditions of drought and re-watering at seedling stage. Chinese Journal of Plant Ecology (植物生态学报), 2014, 38(7): 729-739 (in Chinese)
[17] Shao H-F (邵慧芳), Chen Z (陈征), Xu J-Y (许嘉阳), et al. Physiological responses of two tobacco cultivar leaves to different drought stresses during seedling stage. Plant Physiology Journal (植物生理学报), 2016, 52(12): 1861-1871 (in Chinese)
[18] Monneveux P, Sanchez C, Beck D. Drought tolerance improvement in tropical maize source population. Crop Science, 2006, 46: 180-191
[19] Xie X-Y (谢小玉), Zhang X (张霞), Zhang B (张兵). Evaluation of drought resistance and analysis of variation of relevant parameters at seedling stage of rapeseed (Brassica napus L.). Scientia Agriculturaa Sinica (中国农业科学), 2013, 46(3): 476-485 (in Chinese)
[20] Luo J-J (罗俊杰), Ou Q-M (欧巧明), Ye C-L (叶春雷), et al. Comprehensive valuation of drought resis-tance and screening of indices of important flax cultivars. Acta Agronomica Sinica (作物学报), 2014, 40(7): 1259-1273 (in Chinese)
[21] Chen L-T (陈蕾太), Sun A-Q (孙爱清), Yang M (杨敏), et al. Seed vigor evaluation based on adversity resistance index of wheat seed germination under stress conditions. Chinese Journal of Applied Ecology (应用生态学报), 2016, 41(5): 570-576 (in Chinese)
[22] Zhang Y-J (张宇君), Zhao L-L (赵丽丽), Wang P-C (王普昶), et al. Construction and comprehensive eva-luation of drought resistance index system in oatmea during germination. Journal of Nuclear Agricultural Sciences (核农学报), 2017, 31(11): 2236-2242 (in Chinese)
[23] Tian S-J (田山君), Yang S-M (杨世民), Kong F-L (孔凡磊), et al. Screening in southwest China of drought-resistant varieties of maize at the seedling stage. Acta Prataculturae Sinica (草业学报), 2014, 23(1): 50-57 (in Chinese)
[24] Liu G-H (刘光辉), Chen Q-J (陈全家), Wu P-H (吴鹏昊), et al. Screening and comprehensive evaluation of drought resistance indices of cotton at blossing and boll-forming stages. Journal of Plant Genetic Resources (植物遗传资源学报), 2016, 17(1): 53-62 (in Chinese)
[25] Du M-F (杜明凤), Ding G-J (丁贵杰), Zhao X-Z (赵熙州). Responses to continuous drought stress and drought resistance of different Masson pine families. Scientia Silvae Sinicae (林业科学), 2017, 53(6): 21-29 (in Chinese)