Effects of salt  and drought stresses on antioxidant system and RbohC and RbohF genes expression in Brassica campestris

doi:10.13287/j.1001-9332.201903.017

Abstract

Abstract: To investigate the effects of salt stress and PEG-6000 simulating drought stress on the active oxygen and antioxidant enzyme activities, as well as the expression level of RbohC and RbohF genes, the seedlings of two Brassica campestris, Longyou 6 and Tianyou 2, were treated with U0126 (a MAPKK inhibitor), DMTU (a H₂O₂ scavenger), as well as DPI and IMD (NADPH oxidase inhibitors). The results showed that under both stresses, H₂O₂ accumulation as well as antioxidant enzyme (SOD, CAT, APX, GR) activities and the expression of RbohC and RbohF genes increased, while O₂^-· accumulation decreased. The O₂^-· accumulation, antioxidant enzyme activity and RbohC and RbohF genes expression in both varieties all significantly decreased. Compared to seedlings with on pretreatment before salt and PEG-6000 simulating drought stress, the accumulation of H₂O₂ decreased in seedlings pretreated with DMTU, DPI and IMD. However, the accumulation of H₂O₂ increased in those pretreated with U0126. Those results indicated that the NADPH oxidase, MAP kinase cascade and H₂O₂ were involved in the regulation of active oxygen production and antioxidant enzyme activity, as well as the expression of RbohC and RbohF under salt stress and drought stress.

Key words: RbohC and RbohF genes, reactive oxygen, Brassica campestris, enzyme activity analysis, MAP kinase

ZHANG Teng-guo, LI Qiao-li, DIAO Zhi-hong, LI Ping, WANG Juan, ZHENG Sheng. Effects of salt and drought stresses on antioxidant system and RbohC and RbohF genes expression in Brassica campestris[J]. Chinese Journal of Applied Ecology, 2019, 30(3): 969-978.

References

[1] Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physio-logy, 2006, 141: 391-396
[2] Parida AK, Das AB, Mohanty P. Defense potentials to NaCl in a mangrove, Bruguiera parviflora: Differential changes of isoforms of some antioxidative enzymes. Journal of Plant Physiology, 2004, 161: 531-542
[3] Pandey S, Nelson DC, Assmann SM. Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis. Cell, 2009, 136: 136-148
[4] Miller G, Shulaev V, Mittler R. Reactive oxygen signaling and abiotic stress. Physiologia Plantarum, 2010, 133: 481-489
[5] Herve C, Tonon T, Collen J, et al. NADPH oxidases in Eukaryotes: Red algae provide new hints! Current Genetics, 2006, 49: 190-204
[6] BaileySerres J, Mittler R. The roles of reactive oxygen species in plant cells. Plant Physiology, 2006, 141: 311, doi: https://doi.org/10.1104/pp.104.900191
[7] Leshem Y, Seri L, Levine A. Induction of phosphatidylinositol 3-kinase-mediated endocytosis by salt stress leads to intracellular production of reactive oxygen species and salt tolerance. Plant Journal, 2010, 51: 185-197
[8] Sakamoto H, Matsuda O, Iba K. ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana. Plant Journal, 2010, 56: 411-422
[9] Nühse TS, Bottrill AR, Jones AM, et al. Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses. Plant Journal, 2010, 51: 931-940
[10] Jiang M, Zhang J. Cross-talk between calcium and reactive oxygen species originated from NADPH oxidase in abscisic acid-induced antioxidant defence in leaves of maize seedlings. Plant, Cell and Environment, 2010, 26: 929-939
[11] Aziz A, Heyraud A, Lambert B. Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea. Planta, 2004, 218: 767-774
[12] Zhang T-G (张腾国), Nie T-T (聂婷婷), Sun W-C (孙万仓), et al. Effects of diverse stresses on gene expression and enzyme activity of glutathione reductase in Brassica campestris. Chinese Journal of Applied Ecology (应用生态学报), 2018, 29(1): 213-222 (in Chinese)
[13] Sun W-C (孙万仓), Ma W-G (马卫国), Lei J-M (雷建民), et al. Study on adaptation and introduction possibility of winter rapeseed to dry and cold areas in Northwest China. Scientia Agricultura Sinica (中国农业科学), 2007, 40(12): 2716-2726 (in Chinese)
[14] Scarpeci TE, Zanor MI, Carrillo N, et al. Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: A focus on rapidly induced genes. Plant Molecular Biology, 2008, 66: 361-378
[15] Gao X, Chen X, Lin W, et al. Bifurcation of Arabidopsis NLR immune signaling via Ca²⁺-dependent protein kinases. PLoS Pathogens, 2013, 9: e1003127
[16] Sergiev I, Alexieva V, Karanov E. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comptes Rendus de l’Academie Bulgare des Sciences, 1997, 51: 121-124
[17] Ries SK. In vitro production of superoxide radical from paraquat and its interactions with monuron and diuron. Weed Science, 1977, 25: 298-303
[18] Aebi H. Catalase in vitro. Methods in Enzymology, 1984, 105: 121-126
[19] Esterbauer H, Grill D. Seasonal variation of glutathione and glutathione reductase in needles of Picea abies. Plant Physiology, 1978, 61: 119-121
[20] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 1981, 22: 867-880
[21] Mittler R, Vanderauwera S, Gollery M, et al. Reactive oxygen gene network of plants. Trends in Plant Science, 2004, 9: 490-498
[22] Hu X, Jiang M, Zhang J, et al. Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H₂O₂ production in leaves of maize (Zea mays) plants. New Phytologist, 2007, 173: 27-38
[23] Li G-Q (李国旗), An S-Q (安树青), Zhang J-L (张纪林), et al. Impact of salt stress on peroxidase activity in Populus deltoides cambium and its consequence. Chinese Journal of Applied Ecology (应用生态学报), 2003, 14(6): 871-874 (in Chinese)
[24] Chen J (陈珺), Lai Q-X (赖齐贤), He B-L (何宝龙), et al. Production of activated oxygen and antioxidant enzyme activity in ornamental species under drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2014, 34(7): 1390-1396 (in Chinese)
[25] Vaidyanathan H, Sivakumar P, Chakrabarty R, et al. Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.): Differential response in salt-tolerant and sensitive varieties. Plant Science, 2003, 165: 1411-1418
[26] Schopfer P, Plachy C, Frahry G. Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiology, 2001, 125: 1591-1602
[27] Leshem Y, Seri L, Levine A. Induction of phosphatidylinositol 3-kinase-mediated endocytosis by salt stress leads to intracellular production of reactive oxygen species and salt tolerance. Plant Journal, 2010, 51: 185-197
[28] Gupta DK, Inouhe M, Rodríguez-Serrano M, et al. Oxidative stress and arsenic toxicity: Role of NADPH oxidases. Chemosphere, 2013, 90: 1987-1996
[29] Pitzschke A, Hirt H. Disentangling the complexity of mitogen-activated protein kinases and reactive oxygen species signaling. Plant Physiology, 2009, 149: 606-615
[30] Zhang A, Jiang M, Zhang J, et al. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiology, 2006, 141: 475-487
[31] Zhang A, Jiang M, Zhang J, et al. Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves. New Phytologist, 2007, 175: 36-50
[32] Mittler R, Vanderauwera S, Gollery M, et al. Reactive oxygen gene network of plants. Trends in Plant Science, 2004, 9: 490-498
[33] Dat JF, Pellinen R, Beeckman T, et al. Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco. The Plant Journal, 2010, 33: 621-632
[34] Yoshioka H, Numata N, Nakajima K, et al. Nicotiana benthamiana gp91 phox homologs NbrbohA and NbrbohB participate in H₂O₂ accumulation and resistance to Phytophthora infestans. Plant Cell, 2003, 15: 706-718
[35] Zhang H, Liu Y, Feng W, et al. A novel rice C₂H₂-type zinc finger protein, ZFP36, is a key player involved in abscisic acid-induced antioxidant defence and oxidative stress tolerance in rice. Journal of Experimental Botany, 2014, 65: 5795-5809
[36] Kwak JM, Mori IC, Pei ZM, et al. NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis. EMBO Journal, 2014, 22: 2623-2633

Effects of salt and drought stresses on antioxidant system and RbohC and RbohF genes expression in Brassica campestris

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Xin, MA Yingjie, QI Bianbin, YU Bo, LYU Deguo, QIN Sijun. Alleviation effect of GR24, a strigolactone analogue, on low-nitrogen stress in Malus baccata seedlings [J]. Chinese Journal of Applied Ecology, 2023, 34(6): 1592-1600.
[2]	XU Chen-xiao, ZHANG Xiao-yu, LIU Chao-yue, LIU Kun, BI Huan-gai, AI Xi-zhen. Alleviating effect of exogenous melatonin and calcium on the peroxidation damages of cucumber under high temperature stress [J]. Chinese Journal of Applied Ecology, 2022, 33(10): 2725-2735.
[3]	XU Chao, WANG Ming-tian, YANG Zai-qiang, HAN Wei, ZHENG Sheng-hua. Effects of high temperature on photosynthetic physiological characteristics of strawberry seedlings in greenhouse and construction of stress level. [J]. Chinese Journal of Applied Ecology, 2021, 32(1): 231-240.
[4]	YANG Kang, CHEN Jian, XIN Ai-jing, CAI Jin-xia, SHI Zhi-qi, YANG Li-fei. Mechanism of thymol inhibiting Botrytis cinerea: PAO-H₂O₂ system [J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2441-2448.
[5]	NIE Wen-jing, WANG Shuo-shuo, JING Xin, GONG Biao, WEI Min, YANG Feng-juan, LI Yan, SHI Qing-hua. Effects of exogenous 2,4-epibrassinolide on the growth and redox balance of cucumber seedlings under NaHCO₃ stress. [J]. Chinese Journal of Applied Ecology, 2018, 29(3): 899-908.
[6]	ZHANG Teng-guo, NIE Ting-ting, SUN Wan-cang, SHI Zhong-fei, WANG Juan. Effects of diverse stresses on gene expression and enzyme activity of glutathione reductase in Brassica campestris. [J]. Chinese Journal of Applied Ecology, 2018, 29(1): 213-222.
[7]	YANG Sha, HOU Lin-lin, GUO Feng, ZHANG Jia-lei, GENG Yun, MENG Jing-jing,LI Xin-guo, WAN Shu-bo. Effects of exogenous Ca²⁺ on growth and development, physiology and yield of peanut under salt stress [J]. Chinese Journal of Applied Ecology, 2017, 28(3): 894-900.
[8]	RUAN Ya-nan, XU Sheng, GUO Long, ZHU Ming-zhu, WANG Cong, LI Shu-yuan, WANG Hong-yan. Effects of elevated ozone concentrations on reactive oxygen metabolism and related gene expression in Ginkgo biloba leaves [J]. Chinese Journal of Applied Ecology, 2017, 28(11): 3479-3486.
[9]	CHENG Dan-dan, SUN Jian-ping, CHAI Yuan, ZHU Yi-yong, ZHAO Min, SUN Guang-yu, SUN Xing-bin. Effects of Pseudomonas syringae pv. tabaci infection on tobacco photosynthetic apparatus under light or dark conditions. [J]. Chinese Journal of Applied Ecology, 2016, 27(8): 2655-2662.
[10]	GAO Qing-hai, WANG Ya-kun, LU Xiao-min, JIA Shuang-shuang. Effects of exogenous silicon on physiological characteristics of cucumber seedlings under ammonium stress. [J]. Chinese Journal of Applied Ecology, 2014, 25(5): 1395-1400.
[11]	ZHANG Yong-zheng, LI Hai-dong, LI Xiu, XIAO Jing, XU Kun. Effects of water and light interaction on reactive oxygen metabolism in ginger leaves. [J]. Chinese Journal of Applied Ecology, 2013, 24(12): 3459-3464.
[12]	. Effects of desulfurization waste on calcium distribution, Ca2+-ATPase activity, and antioxidant characteristics of rice leaf under alkali stress. [J]. Chinese Journal of Applied Ecology, 2012, 23(02): 363-368.
[13]	. Relationships between reactive oxygen metabolism and endodormancy release of peach bud under short-term heating. [J]. Chinese Journal of Applied Ecology, 2010, 21(11): 2749-2754.
[14]	XIE Guo-sheng¹;SHI Rui-hong^1，2;PANG Zhen-wu¹;CAI Ke-tong¹. Physiological characteristics of rice seedlings roots under aluminum stress. [J]. Chinese Journal of Applied Ecology, 2009, 20(07): 1698-1704 .
[15]	RUAN Ya-nan^1,2;HE Xing-yuan²;CHEN Wei²;CHEN Zhen-ju²; SUN Yu². Effects of elevated O₃ concentration on anti-oxidative enzyme activities in Pinus tabulaeformis. [J]. Chinese Journal of Applied Ecology, 2009, 20(05): 1032-1037 .