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Chinese Journal of Applied Ecology ›› 2019, Vol. 30 ›› Issue (6): 2030-2038.doi: 10.13287/j.1001-9332.201906.038

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Effect of Miscanthus planting on the structure and function of soil bacterial community.

DUAN Peng-fei1, CHEN Yan2, ZHANG Fei2, HAN Hui1, PANG Fa-hu1, CHEN Zhao-jin1,*, TIAN Wei3   

  1. 1School of Agricultural Engineering, Nanyang Normal University/Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Nanyang 473061, Henan, China;
    2School of Life Science and Technology, Nanyang Normal University,Nanyang 473061, Henan, China;
    3Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China
  • Received:2018-08-16 Online:2019-06-15 Published:2019-06-15
  • Supported by:

    This work was supported by National Natural Science Foundation of China (41601332, 31501260), the Key Science and Technology Project of Henan Province (172102110259, 182102110468, 182102110239), and the Key Research Project of Colleges and Universities of Henan Province Education Department (16A210012)

Abstract: Miscanthus is a promising candidate species of second-generation energy crops. To our knowledge, the composition and function of Miscanthus rhizosphere bacterial communities has not yet been reported. In this study, rhizosphere soil samples were collected from Miscanthus (Xiangzamang No. 1) which was grown in Nanyang City for five years and bareground (as the control) to analyze the bacterial community structure and diversity using 16S rRNA gene sequencing on the Illumina MiSeq platform. PICRUSt was used to determine the metabolic and functional abilities of soil bacterial communities. Phylogenetic analysis based on 16S rRNA gene sequences showed that sol bacteria could be divided into 23 phyla and 231 genera, with high richness of the community composition. The dominant phyla were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Bacteroidetes and Gemmatimonadetes. The bacterial community diversity in the rhizosphere samples of Xiangzamang No. 1 was lower than that in unplanted samples. Rhizosphere bacterial communities were affected by Miscanthus crops. Based on the PICRUSt analysis of bacterial community functions, our results revealed a wide genetic diversity of organisms involved in various essential processes,such as amino acid transport and metabolism, cell wall/membrane/envelope biogenesis, signal transduction mechanisms. Based on the 16S rRNA gene copy number of the detected phylotypes, 22 bacterial metabolic function in the rhizosphere samples of Miscanthus was higher than that in bareground. Results from N- and P-cycling gene analysis showed that Miscanthus planting altered the N- and P-cycling metabolic capacities of soil bacteria.