Abstract: Tree seedlings are one of the important components in alpine treeline ecotone, whose regeneration is crucial to treeline migration in response to climate change. We analyzed the spatial distribution, regeneration of tree seedlings and their responses to climate change in treeline ecotone in recent decades. The spatial distribution of tree seedlings in treeline ecotone is dominated by diffuse and clustered forms, with different indicative significance for spatial dynamics of treeline. At global scale, the altitude distribution limits of tree seedlings are usually related to the length and average temperature of growing season, along with the species characteristics. However, precipita-tion plays an important role at regional scale. The initial stage of seedling recruitment is restricted by seed source, which determines seed distribution and germination to a great extent. Microenvironment facilitates seedling regeneration by providing shelter for establishment and improving their survival rate. The regeneration process is more relevant to multiple biotic, abiotic factors and their interactions. With global warming, rising temperature in treeline ecotone and more precipitation are more suitable for seedling regeneration of treeline species. The expansion of seedlings to higher elevations could be considered as the portent of timberline upwards migration. Due to species-specific adaption strategy, however, some trees only increase seedling density and timberline location is constant. In the future, it is necessary to take precise dating techniques, such as tree-ring and ¹⁴C dating, and conduct long-term in-situ monitoring and indoor simulation experiments. To provide scientific basis for mountain ecosystem restoration and conservation, we should strengthen the studies on spatial patterns and regeneration mechanism of seedlings in treeline ecotone at multiple spatio-temporal scales, the adaptation strategies of tree seedlings in different types of treeline ecotone and treeline dynamics prediction.