Abstract: This study analyzed the sensitivities of three vegetation biochemical parameters \[chlorophyll content (Cab), leaf water content (Cw), and leaf area index (LAI)\] to the changes of canopy reflectance, with the effects of each parameter on the wavelength regions of canopy reflectance considered, and selected three vegetation indices as the optimization comparison targets of cost function. Then, the Cab, Cw, and LAI were estimated, based on the particle swarm optimization algorithm and PROSPECT+SAIL model. The results showed that the retrieval efficiency with vegetation indices as the optimization comparison targets of cost function was better than that with all spectral reflectance. The correlation coefficients (R²) between the measured and estimated values of Cab, Cw, and LAI were 90.8%, 95.7%, and 99.7%, and the root mean square errors of Cab, Cw, and LAI were 4.73 μg·cm^-2, 0.001 g·cm^-2, and 0.08, respectively. It was suggested that to adopt- vegetation indices as the optimization comparison targets of cost function could effectively improve the efficiency and precision of the retrieval of biochemical parameters based on PROSPECT+SAIL model.