Characteristics of Leontopodium leontopodioides leaf stochiometry with altitude and their relationship with soil nutrients in Qilian Mountains, Northwest China

doi:10.13287/j.1001-9332.201912.009

Abstract

Abstract: Foliar stoichiometry provides information on the biotic and abiotic changes of environment. We examined the stoichiometric characteristics of plant leaves at different altitudes to understand how plants adapt to environmental changes. Foliar stoichiometry of Leontopodium leontopodioides at various altitudes (2400, 2600, 2800, 3000 and 3200 m) were analyzed in the Qilian Mountains of China. Across the altitude gradient, mean value of leaf carbon content (LC), nitrogen content (LN), and phosphorous content (LP) of L. leontopodioides was 401.27, 23.99 and 1.22 g·kg^-1, respectively. The mean value of LC:LN, LC:LP and LN:LP was 16.8, 352.5 and 20.7, respectively. LC, LC:LN, LC:LP and LN:LP initially increased with increases in altitude, rea-ching the maximum at 2600 m, then decreased, reaching the minimum at 3000 m, and finally increased again. LP exhibited the opposite trend. LN demonstrated an initial decrease with altitude, reaching the minimum at 2800 m, followed by an increase at higher altitudes. LC did not correlate with LN, but was significantly negatively correlated with LP. LN was significantly positively correlated with LP. There was no correlation between LNand any other stoichiometry ratios. LP showed a significantly negative correlation with other stoichiometry ratios. LC:LN, LC:LP, and LN:LP were positively correlated with each other. Both soil total nitrogen and total phosphorus affected LC and LN, whereas LP was significantly negatively correlated with soil total phosphorus. The results suggested that the growth of L. leontopodioides in the study region was mainly limited by P availability.

ZHANG Xiao-fang, LIU Xian-de, JING Wen-mao, CAO Jian-jun. Characteristics of Leontopodium leontopodioides leaf stochiometry with altitude and their relationship with soil nutrients in Qilian Mountains, Northwest China[J]. Chinese Journal of Applied Ecology, 2019, 30(12): 4012-4020.

References

[1] Elser JJ, Fagan WF, Kerkhoff AJ, et al. Biological stoichiometry of plant production: Metabolism, scaling and ecological response to global change. New Phytologist, 2010, 186: 593-608
[2] Yang X, Chi X, Ji C, et al. Variations of leaf N, P concentrations in shrubland biomes across northern China: Phylogeny, climate and soil. Biogeosciences, 2016, 12: 18973-18998
[3] Vitouseck PM. Nutrient cycling and nutrient use efficiency. American Naturalist, 1982, 119: 553-572
[4] Wu T-G (吴统贵), Chen B-F (陈步峰), Xiao Y-H (肖以华), et al. Leaf stoichiometry of trees in three forest types in Pearl River Delta, South China. Chinese Journal of Plant Ecology (植物生态学报), 2010, 34(1): 58-63 (in Chinese)
[5] He JS, Wang L, Flynn DF, et al. Leaf nitrogen: Phosphorus stoichiometry across Chinese grassland biomes. Oecologia, 2008, 155: 301-310
[6] Qin Y-Y (秦燕燕), Feng Q (冯起), Zhu M (朱猛), et al. Influence of slope aspect on ecological stoichiometry of grassland plant leaves in Dayekou Basin of Qilian Mountains. Journal of Lanzhou University (Nature Science) (兰州大学学报:自然科学版), 2017, 53(3): 362-367 (in Chinese)
[7] Pi F-J (皮发剑), Yuan C-J (袁从军), Yu L-F (喻理飞), et al. Ecological stoichiometry characteristics of plant leaves from the main dominant species of natural secondary forest in the central of Guizhou. Ecology and Environmental Sciences (生态环境学报), 2016, 25(5): 801-807 (in Chinese)
[8] Hong J-T (洪江涛), Wu J-B (吴建波), Wang X-D (王小丹). Effects of global climate change on the C, N, and P stoichiometry of terrestrial plants. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(9): 2658-2665 (in Chinese)
[9] Redfield AC. The biological control of chemical factors in the environment. Science Progress, 1960, 11: 150-170
[10] Zechmeister-Boltenstern S, Keiblinger KM, Mooshammer M, et al. The application of ecological stoichiometry to plant-microbial-soil organic matter transformations. Ecological Monographs, 2015, 85: 133-155
[11] Luo Y (罗艳), Gong L (贡璐), Zhu M-L (朱美玲), et al. Stoichiometry characteristics of leaves and soil of four shrubs in the upper reaches of the Tarim River Desert. Acta Ecologica Sinica (生态学报), 2017, 37(24): 8326-8335 (in Chinese)
[12] Lin Y-J (林永静), Wu M-J (武梦娟), Lu T-P (卢同平), et al. The visualization analysis of the ecological stoichiometry research based on the CiteSpace. Journal of Biology (生物学杂志), 2018, 35(2): 63-66 (in Chinese)
[13] Han WX, Guo DL, Zhang Y. Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China. New Phytologist, 2005, 168: 377-385
[14] Xia C, Yu D, Wang Z, et al. Stoichiometry patterns of leaf carbon, nitrogen and phosphorous in aquatic macrophytes in eastern China. Ecological Engineering, 2014, 70: 406-413
[15] Xu X-Y (许雪贇), Qin Y-Y (秦燕燕), Cao J-J (曹建军). Characteristics of ecological stoichiometry of Potentilla chinensis leaves in the northeastern Qinghai-Tibet Plateau with altitude. Acta Ecologica Sinica (生态学报), 2019, 39(24): doi: 10.5846/stxb201805231128 (in Chinese)
[16] Sardans J, Rivas Ubach A, Peñuelas J. The C:N:P stoichiometry of organisms and ecosystems in a changing world: A review and perspectives. Perspective in Plant Ecology, Evolution and Systematics, 2012, 14: 33-47
[17] Wang L, Zhao G, Li M, et al. C:N:P stoichiometry and leaf traits of halophytes in an arid saline environment, northwest China. PLoS One, 2015, 10(3): e0119935
[18] Shi Z-M (史作民), Cheng R-M (程瑞梅), Liu S-R (刘世荣). Response of leaf δ¹³C to altitudinal gradients and its mechanism. Acta Ecologica Sinica (生态学报), 2004, 24(12): 2901-2906 (in Chinese)
[19] Jin M (金铭), Li Y (李毅), Liu X-D (刘贤德), et al. Spatial structure characteristic of Picea crassifolia in Qilian Mountains. Arid Land Geography (干旱区地理), 2012, 35(4): 587-593 (in Chinese)
[20] Zhu M (朱猛), Liu W (刘蔚), Qin Y-Y (秦燕燕), et al. Distribution of soil organic carbon at hill slope scale in forest-steppe zone of Qilian Mountains. Journal of Desert Research (中国沙漠), 2016, 36(3): 741-748 (in Chinese)
[21] He T (何涛), Wu X-M (吴学明), Wang X-R (王学仁), et al. Photosynthetic characteristics of Leontopodium leontopodioides growing at different altitudes. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 2005, 25(12): 2519-2523 (in Chinese)
[22] Mu X-M (牟晓明), Yu Y-W (于应文), Wang X-Z (王先之), et al. Analysis of community spatial patterns of Leontopodium nanum patches in Qinghai-Tibetan Pla-teau. Acta Ecologica Sinica (生态学报), 2015, 35(16): 5306-5315 (in Chinese)
[23] Wu Y-W (武彦文), Gao W-Y (高文远), Su Y-F (苏艳芳), et al. Phytochemcial and pharmacological research progress in Leontopodium medicinal plants. China Journal of Chinese Materia Medica (中国中药杂志), 2005, 30(4): 245-248 (in Chinese)
[24] Sun H-T (孙会婷), Jiang S (江莎), Liu J-M (刘婧敏), et al. Structure and ecological adaptability of the leaves of three Asteraceae species at different altitudes on the Qinghai-Tibet Plateau. Acta Ecologica Sinica (生态学报), 2016, 36(6): 1559-1570 (in Chinese)
[25] Qi P (齐鹏), Liu X-D (刘贤德), Zhao W-J (赵维俊), et al. Evaluation of soil fertility in Picea crassifolia forest in Dayekou basin of Qilian Mountains. Grassland and Turf (草原与草坪), 2015, 35(4): 37-43 (in Chinese)
[26] Zhu M, Feng Q, Zhang M, et al. Soil organic carbon in semiarid alpine regions: The spatial distribution, stock estimation, and environmental controls. Journal of Soils and Sediments, 2019, 19: 3427-3441
[27] Elser JJ, Fagan WF, Denno RF, et al. Nutritional constraints in terrestrial and freshwater food webs. Nature, 2000, 408: 578-580
[28] Zheng S-X (郑淑霞), Shangguan Z-P (上官周平). Spatial distribution pattern of plant nutrient composition in the Loess Plateau. Progress in Natural Science (自然科学进展), 2006, 16(8): 965-973 (in Chinese)
[29] Yang S-Q (杨思琪), Zhao X-J (赵旭剑), Sen D (森道), et al. Leaf C, N and P chemometries and their altitudinal variations in the central Tianshan Mountains. Arid Zone Research (干旱区研究), 2017, 34(6): 1371-1379 (in Chinese)
[30] Zhao W-J (赵维俊), Liu X-D (刘贤德), Jin M (金铭), et al. Ecological stoichiometric characteristics of carbon, nitrogen and phosphorus in leaf-litter-soil system of Picea crassifolia forest in the Qilian Mountains. Acta Pedologica Sinica (土壤学报), 2016, 53(2): 477-489 (in Chinese)
[31] Yan X (严雪), Yu D (于丹), Li Y-K (李永科). Response of growth rate and nutrient elements accumulation in submerged clonal macrophyte under elevated CO₂. Chinese Journal of Plant Ecology (植物生态学报), 2003, 27(4): 435-440 (in Chinese)
[32] He H-L (贺合亮), Yang X-C (阳小成), Li D-D (李丹丹), et al. Stoichiometric characteristics of carbon, nitrogen and phosphorus of Sibiraea angustata shrub on the eastern Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology (植物生态学报), 2017, 41(1): 126-135 (in Chinese)
[33] Xu X-Y (许雪贇), Qin Y-Y (秦燕燕), Cao J-J (曹建军), et al. Elevational variations of leaf stochiometry in Leontopodium leontopodioides on the Qinghai-Tibetan Plateau, China. Chinese Journal of Applied Ecology (应用生态学报), 2018, 29(12): 3934-3940 (in Chinese)
[34] Fan J, Harris W, Zhong H. Stoichiometry of leaf nitrogen and phosphorus of grasslands of the Inner Mongolian and Qinghai-Tibet plateau in relation to climatic variables and vegetation organization levels. Ecological Research, 2016, 31: 821-869
[35] Zhao N, He N, Wang Q, et al. The altitudinal patterns of leaf C:N:P stoichiometry are regulated by plant growth form, climate and soil on Changbai Mountain, China. PLoS One, 2014, 9(4): e95196
[36] Reich PB, Oleksyn J. Global patterns of plant leaf N and P in relation to temperature and latitude. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101: 11001-11006
[37] Oleksyn J, Modrzynski J, Tjoelker MG, et al. Growth and physiology of Picea abies populations from elevational transects: Common garden evidence for altitudinal ecotypes and cold adaptation. Functional Ecology, 1998, 12: 573-590
[38] Weih M, Karlsson PS. Growth response of mountain birch to air and soil temperature: Is increasing leaf-nitrogen content an acclimation to lower air temperature? New Phytologist, 2001, 150: 147-155
[39] Reich PB, Walters MB, Ellsworth DS, et al. Relationships of leaf dark respiration to leaf nitrogen, specific leaf area and leaf life-span: A test across biomes and functional groups. Oecologia, 1998, 114: 471-482
[40] Song L-L (宋璐璐), Fan J-W (樊江文), Wu S-H (吴绍洪), et al. Response characteristics of leaf traits of common species along an altitudinal gradient in Hongchiba Grassland, Chongqing. Acta Ecologica Sinica (生态学报), 2012, 32(9): 2759-2767 (in Chinese)
[41] Li ZQ, Yang L, Lu W, et al. Spatial patterns of leaf carbon, nitrogen stoichiometry and stable carbon isotope composition of Ranunculus natans, C.A. Mey. (Ranunculaceae) in the arid zone of northwest China. Ecological Engineering, 2015, 77: 9-17
[42] Sardans J, Rivas-Ubach A, Peñuelas J. Factors affecting nutrient concentration and stoichiometry of forest trees in Catalonia (NE Spain). Forest Ecology and Management, 2011, 262: 2024-2034
[43] Ågren GI. Stoichiometry and nutrition of plant growth in natural communities. Annual Review of Ecology, Evolution, and Systematics, 2008, 39: 153-170
[44] Matzek V, Vitousek PM. N:P stoichiometry and protein:RNA ratios in vascular plants: An evaluation of the growth-rate hypothesis. Ecology Letters, 2009, 12: 765-771
[45] Niklas KJ. Plant allometry, leaf nitrogen and phosphorus stoichiometry, and interspecific trends in annual growth rates. Annals of Botany, 2006, 97: 155-163
[46] Koerselman W, Meuleman AFM. The vegetation N:P ratio: A new tool to detect the nature of nutrient limitation. Journal of Applied Ecology, 1996, 33: 1441-1450
[47] Niu D-C (牛得草). Leaf Carbon, Nitrogen and Phosphorus Stoichiometry across Plant Species on the Wes-tern Slopes of Helan Mountain. PhD Thesis. Lanzhou: Lanzhou University, 2011 (in Chinese)
[48] Xu X-Y (许雪贇), Cao J-J (曹建军), Yang L (杨淋), et al. Effects of grazing and enclosure on foliar and soil stoichiometry of grassland on the Qinghai-Tibetan Plateau. Chinese Journal of Ecology (生态学杂志), 2018, 37(5): 1349-1355 (in Chinese)
[49] Wang Z-F (汪宗飞), Zheng F-L (郑粉莉). C, N, and P stoichiometric characteristics of Pinus tabuliformis plantation in the Ziwuling Region of the Loess Plateau. Acta Ecologica Sinica (生态学报), 2018, 38(19): 87-97 (in Chinese)
[50] Zhou XB, Matthew A, Tao Y, et al. Chronic nitrogen addition induces a cascade of plant community responses with both seasonal and progressive dynamics. Science of the Total Environment, 2018, 626: 99-108
[51] Bin Z-J (宾振钧), Zhang R-Y (张仁懿), Zhang W-P (张文鹏), et al. Effects of nitrogen, phosphorus and silicon addition on leaf carbon, nitrogen, and phosphorus concentration of Elymus nutans of alpine meadow on Qinghai-Tibetan Plateau, China. Acta Ecologica Sinica (生态学报), 2015, 35(14): 4699-4706 (in Chinese)
[52] Yan K (阎凯), Fu D-G (付登高), He F (何峰), et al. Leaf nutrient stoichiometry of plants in the phosphorus-enriched soils of the Lake Dianchi watershed, southwestern China. Chinese Journal of Plant Ecology (植物生态学报), 2011, 35(4): 353-361 (in Chinese)
[53] Jiang J (蒋婧), Song M-H (宋明华). Review of the roles of plants and soil microorganisms in regulating ecosystem nutrient cycling. Chinese Journal of Plant Ecology (植物生态学报), 2010, 34(8): 979-988 (in Chinese)
[54] Ding F (丁凡), Lian P-Y (廉培勇), Zeng D-H (曾德慧). Characteristics of plant leaf nitrogen and phosphorus stoichiometry in relation to soil nitrogen and phosphorus concentrations in Songnen Plain meadow. Chinese Journal of Ecology (生态学杂志), 2011, 30(1): 77-81 (in Chinese)
[55] Zhu M, Feng Q, Qin Y, et al. Soil organic carbon as functions of slope aspects and soil depths in a semiarid alpine region of northwest China. Catena, 2017, 152: 94-102
[56] Chang X-X (常学向), Zhao W-Z (赵文智), Zhao A-F (赵爱芬). Species diversity of pasture community at different altitude levels in Qilian Mountains. Chinese Journal of Applied Ecology (应用生态学报), 2004, 15(9): 1599-1603 (in Chinese)