Abstract: A simulated field experiment with three treatments, i.e., ambient light (control), reduced UV-B radiation, and enhanced UV-B radiation, was conducted to evaluate the effects of solar ultraviolet (UV-B) radiation on the seedlings of Myrica rubra, a typical woody species in subtropical region. The leaf chlorophyll content, spectral reflectance and spectral characteristic parameters were measured and analyzed. As compared with the control, enhanced UV-B radiation decreased the seedling chlorophyll content while reduced UV-B radiation significantly increased the chlorophyll content, and these effects reflected in the spectral reflectance. Under the effects of the three gradients of UV-B radiation, the differences in the reflectance at visible region mainly occurred around the green peak and red edge on the reflectance curve, and the peak wavelength of the red edge shifted to longer wavelength. Enhanced UV-B radiation had an accumulated temporal effect on M. rubra. The invertedGaussian model parameters R₀,_λ₀,λ_p,R_s, and б were the useful guides to reveal the spectral responses of M. rubra seedlings under UV-B radiation stress, among which, R_s performed the best. The differences in the spectral reflectance under different UV-B radiation levels could be effectively distinguished with the vegetation indices composed of the spectral reflectance of narrow wave bands or the reflectance at specific wavelengths.