Abstract: Based on the identification of the threatened plants in Northeast China and the priority conservation value of plants, the priority conservation regions of the threatened plants in Northeast China were determined. In the study area, there were 60 species of threatened plants, belonging to 42 genera and 25 families. In the 219 counties (or cities) of Northeast China, 119 counties (or cities) had the distribution of threatened plants. The Antu County in Jilin Province had the most species (42) of threatened plants. A total of 16 counties (cities) such as the Antu County of Jilin Province and the Huanren Manchu Autonomous County of Liaoning Province, etc. were identified as the priority conservation regions of the threatened plants in Northeast China. According to the priority conservation value, five priority conservation regions of threatened plants in Northeast China were divided, including Changbai Mountain conservation region, East Liaoning conservation region, South Liaoning conservation region, Zhangguangcai Mountain conservation region, and Xiaoxing’an Mountain conservation region. The main threatened plants in each priority conservation region were also analyzed.