Abstract: By using the heritability-adjusted GGE biplot analysis method, and taking the trial sites Anqing, Nanyang, Huanggang, Jingzhou, Wuhan, Xiangyang, Changde, Yueyang, Nanjing, Nantong, Yancheng, Jiujiang, Jianyang, Shehong, and Cixi as the representative cotton-planting areas in the Yangtze River basin, the ecological regionalization of cotton varieties in the basin was made based on the lint cotton yield, and the regionalization results were adjusted by the information ratio (IR) method, aimed to provide scientific basis for the selection of cotton varieties in the cottonplanting areas of the basin. The cottonplanting areas in the Yangtze River basin could be divided into three ecological regions, i.e., the “Sichuan basin cotton region” with Jianyang and Shehong as the representative, the “NanXiang basin cotton region” with Xiangyang and Nanyang as the representative, and the “majority complex cotton region in the Yangtze River basin” including all the other sites in the basin.