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Molecular identification and detection of moon jellyfish (Aurelia sp.) based on partial sequencing of mitochondrial 16S rDNA and COI.

WANG Jian-yan1, ZHEN Yu2, WANG Guo-shan1, MI Tie-zhu2, YU Zhi-gang3   

  1. (1College of Marine Life Sciences, Ocean University of China, Qingdao 266003, Shandong, China; 2Ministry of Education Key Laboratory of Marine Environment and Ecology, Ocean University of China, Qingdao 266100, Shandong, China; 3Ministry of Education Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Qingdao 266100, Shandong, China)
  • Online:2013-03-18 Published:2013-03-18

Abstract: Taking the moon jellyfish Aurelia sp. commonly found in our coastal sea areas as test object, its genome DNA was extracted, the partial sequences of mt-16S rDNA (650 bp) and mt-COI (709 bp) were PCR-amplified, and, after purification, cloning, and sequencing, the sequences obtained were BLASTnanalyzed. The sequences of greater difference with those of the other jellyfish were chosen, and eight specific primers for the mt-6S rDNA and mt-COI of Aurelia sp. were designed, respectively. The specificity test indicated that the primer AS3 for the mt16S rDNA and the primer AC3 for the mtCOI were excellent in rapidly detecting the target jellyfish from Rhopilema esculentum, Nemopilema nomurai, Cyanea nozakii, Acromitus sp., and Aurelia sp., and thus, the techniques for the molecular identification and detection of moon jellyfish were preliminarily established, which could get rid of the limitations in classical morphological identification of Aurelia sp., being able to find the Aurelia sp. in the samples more quickly and accurately.