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Chinese Journal of Applied Ecology ›› 2020, Vol. 31 ›› Issue (3): 761-768.doi: 10.13287/j.1001-9332.202003.020

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Difference of soil nematode communities between the humus and soil surface layer in the cold temperate coniferous and broadleaved mixed forest of Yulong Snow Mountain Nature Reserve, Yunnan, China

WANG Wen-ting1,2, XIA Shang-wen2, XIAO Hai-feng3, LIU Sheng-jie2, YANG Xiao-dong2*   

  1. 1University of Chinese Academy of Sciences, Beijing 100049, China;
    2Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan, China;
    3Xiangnan University, Chenzhou 423099, Hunan, China
  • Received:2019-09-17 Online:2020-03-15 Published:2020-03-15
  • Contact: E-mail: yangxd@xtbg.ac.cn
  • Supported by:
    This work was supported by the Chinese Academy of Sciences 135 Program (2017XTBG-T01) and the Program of Southeast Asia Biodiversity Research Center, Chinese Academy of Sciences (Y4ZK111B01)

Abstract: A high-throughput sequencing approach was used to differentiate the nematode communities in the humus and soil surface layer (0-10 cm) in 20 hm2 plot located in the cold temperate coniferous and broadleaved mixed forest of Yulong Snow Mountain Nature Reserve, Lijiang, Yunnan. A total of 5744582 sequences were obtained, which were further annotated to 44 nematode families. In the humus layer, 37 families were recorded, with Tylenchidae (18.1%) being the most dominant family. For trophic groups, bacterivorous, fungivorous and herbivorous were predominant. The soil surface layer had 41 families, with Mononchidae (45.4%) being the most dominant family. The relative abundance of predatory nematode was highest in the soil surface layer. There was no significant difference in the α diversity indices (Shannon, Simpson and Chao1 index) of nematode communities at the OTU level between two habitats. At the family level, however, α diversity of nematode community in the humus layer was significantly lower than in soil surface layer. β diversity of the nematode community was significantly different in the two habitats, with lower Cody index but high Sorensen non-similarity index in the humus habitat. Results of non-metric multidimensional scaling (NMDS) analysis showed that the internal structure difference of nematode community in the soil surface layer was higher than that in the humus layer.