Abstract: As an ecological protected area for rare animals and plants in a subtropical forest zone, Shennongjia National Reserve plays an important role in the study of biodiversity in China. By using the market value, shadeprice and opportunitycost methods, the forest ecosystem service values of Shennongjia National Nature Reserve were evaluated, including forest production, recreation and culture, water conservation, soil conservation, gas regulation, environment purification, nutrient circulation and biodiversity conservation. The total value of the Shennongjia Nature Reserve was approximately 204.33×10⁸ yuan RMB·a^-1. The values of the different functions were in order of biodiversity conservation (68.5%)> soil conservation (12.7%)> recreation and culture (4.9%)>gas regulation (4.8%)>forest production (4.2%)> water conservation (3.9%)> environment purification (0.7%)>nutrient circulation (0.3%). The values with respect to utility were in sequence of unused value (68.5%)> indirect value in use (22.4%)> direct value in use (9.1%).