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Bacterial community structure and diversity in soils of different forest ages and types in Baotianman forest, Henan Province, China.

BAI Xiao-xu1,2, SHI Rong-jiu1, YOU Ye-ming3, SHENG Hua-fang4, HAN Si-qin1, ZHANG Ying1   

  1. (1Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2University of Chinese Academy of Sciences, Beijing 100049, China; 3Ministry of Education Key Laboratory for Silvia Culture and Conservation, College of Forest Science, Beijing Forestry University, Beijing 100083, China; 4Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China)
  • Online:2015-08-18 Published:2015-08-18

Abstract: To compare the microbial compositions and diversities in soils of different forest ages and types in Baotianman forest, Henan Province, China, genomic DNA of forest soils was extracted for amplifying the 16S rRNA V4 hyper variable region by PCR and sequencing by Illumina MiSeq. The BIPES, UCHIME and QIIME were employed to analyze the soil bacterial community. It was shown that 60 phyla were identified, with Proteobacteria, Acidobacteria, and Verrucomicrobia representing the most dominant lineages and accounting for 29%, 18.5% and 10% of all sequences, respectively. At the genus level, 1209 genera were identified, the most abundant phylotypes were DA101(6.3%), Acidobacteria2 (5.9%), Candidatus Solibacter (2.9%) and Candidatus Nitrososphaera(2.6%). Different forest age and type soil samples had unique compositions and specific high and rare genus. Forest type and age both impacted the soil microbial community structure, and the influence of the former was stronger than the latter. The soil microbial diversity of the 80yearold  Quercus aliena forest was the lowest among all age and type forest soil samples. Soil pH, soil nitrogen and organic carbon contents were the most important factors affecting soil bacterial community structure.