Abstract: Through in situ observation and indoor tests, the hydrochemical characteristics of a typical karst watershed at three different time scales (diurnal, single storm, and seasonal scales) from June 2013 to March 2014 were investigated, and their influencing factors were analyzed. The results showed that the diurnal variations of the hydrochemistry exhibited a regular changing pattern resulting from the shifting of the main vegetation physiological activity from photosynthesis in the day to respiration in the night. At single storm scale, however, the hydrochemical processes were mainly determined by the number of consecutive rainless days and rainfall intensity, while the diurnal scale effect was weakened. As to the seasonal scale, the overall hydrochemical processes showed quick responses to rainfall events although they responded more quickly in the rainy season than in the dry season. The temperature and the yearly rainfall distribution regime were the two main influencing factors at this scale.