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Effects of high temperature on Bt protein content and nitrogen metabolic physiology in boll wall of Bt cotton.

WANG Jun1, Eltayib H.M.A. Abidallah1,2, HUA Ming-ming1, HENG Li1, LYU Chun-hua1, CHEN De-hua1   

  1. (1Jiangsu Province Key Laboratory of Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, China; 2Faculty of Forestry, Department of Forest Management, Khartoum University, Khartoum 13314, Sudan)
  • Online:2015-10-18 Published:2015-10-18

Abstract: Bt cotton cultivar Sikang 1 (a conventional cultivar) and Sikang 3 (a hybrid cultivar) from China, and 99B (a conventional cultivar) and Daiza 1 (a hybrid cultivar) from USA were selected as experimental materials, the ball wall Bt protein content and nitrogen metabolic physiology were investigated under different high temperature levels at peak boll stage. The results showed that the Bt protein content of boll wall decreased with the increasing temperature. Compared with the control (32 ℃), the boll wall Bt protein content decreased significantly when the temperature was above 38 ℃ for the conventional cultivars and above 40 ℃ for the hybrid cultivars. The Bt protein contents of cultivar Sikang 1 and 99B decreased by 53.0% and 69.5% respectively with the temperature at 38 ℃, and that of cultivar Sikang 3 and Daiza 1 decreased by 64.8% and 54.1% respectively with the temperature at 40 ℃. Greater reductions in the boll wall soluble protein contents and GPT activities, larger increments for the boll wall free amino acid contents and proteinsase activities were also observed when the boll wall Bt protein content was significantly reduced. Therefore, high temperature resulted in the reduction of Bt protein synthesis and increase of the insecticidal protein degradation in the boll wall significantly, which caused the reductions in boll wall Bt protein content and insect resistance.