Abstract: Land use change is the core content of global change. To achieve sustainable land use planning, it is necessary to evaluate the habitat quality pattern and its spatiotemporal variation resulted from land use change, which can provide basis for the formulation of land management policy. Based on the analysis of land use change from 2000 to 2010, this study investigated the spatio-temporal variation of habitat quality pattern of BeijingTianjinHebei Area. We used the watershed profile sampling points and spatial autocorrelation analysis based on watershed subdivision. The results showed that the main land use change types from 2000 to 2010 in this area included the transition from cultivated land to construction land, the transition between forest and grassland, and the transition from water bodies to cultivated land. This land use/cover change process led to the decrease of heterogeneity of landscape structure and increase of fragmentation. The overall spatial pattern of habitat quality was that southeast and south areas were relatively lower, while north and west areas were relatively higher. The analysis based on watershed profile showed that the habitat quality of each watershed presented significant difference in each part. Habitat quality of most sampling points degraded in a way, while some improved compared with 2000. In general, the habitat quality of the bottom part of Luanhe River basin, the medium part of BaiChaobaiChaobaixin River basin, the medium and the bottom part of Yongding River basin and medium part of LaozhangFudongpaiBeipai River basin were poor and volatile, while other parts were relatively good. There was a decreasing agglomeration characteristic of distribution of habitat quality in BeijingTianjinHebei Area under the disturbance of human activities. Areas of high habitat quality in 2000 were mainly located in Luanhe River basin and top part of Baihe basin. Areas of low habitat quality were mainly located in medium and bottom part of Yongding River basin and some parts of Chaobai River basin. However, the areas of high habitat quality and areas with low habitat quality of 2010 had both expanded towards southwest.