Abstract: Field experiments were conducted during 2012-2014 wheat growing seasons. With no irrigation in the whole stage (W₀) treatment as control, three supplemental irrigation treatments were designed based on average relative soil moisture contents at 0-140 cm layer, at jointing and anthesis stages (65% for treatment W₁; 70% for treatment W₂; 75% for treatment W₃; respectively), to examine effects of supplemental irrigation on nitrogen accumulation and translocation, grain yield, water use efficiency, and soil nitrate nitrogen leaching in wheat field. Soil water consumption amount, the percentage of soil water consumption and water irrigation to total water consumption in W₂ were higher, and soil water consumption of W₂ in 100-140 cm soil layer was also higher. The nitrogen accumulation before anthesis and after anthesis were presented as W₂, W₃＞W₁＞W₀, the nitrogen accumulation in vegetative organs at maturity as W₃＞W₂＞W₁＞W₀, and the nitrogen translocation from vegetative organs to grain and the nitrogen accumulation in grain at maturity as W₂＞W₃＞W₁＞W₀. At maturity,  soil NO₃^--N content in 0-60 cm soil layer was presented as W₀＞W₁＞W₂＞W₃, that in 80-140 cm soil layer was significantly higher in W₃  than in the other treatments, and no significant difference was found in 140-200 cm  soil layer among all treatments. W₂ treatment  obtained the highest grain yield, water use efficiency, nitrogen uptake efficiency and partial productivity of applied nitrogen. As far as grain yield, water use efficiency, nitrogen uptake efficiency and soil NO₃^--N leaching were concerned, the W₂ regime was the optimal irrigation treatment in this experiment.