Multiple resistance selection with insecticides and change of detoxification enzyme activities in Tetranychus turkestani Ugarov et Nikolski (Acari: Tetranychidae).

doi:10.13287/j.1001-9332.201709.003

Abstract

Abstract: In order to explore the multi-pesticide resistance and biochemical mechanism of Tetranychus turkestani, the related susceptible strains (SS) of T. turkestani were treated by mixture of spirodiclofen, fenpropathrin and abamectin to breed multiple resistance strains (Mp-R) in the laboratory. The results showed that the multiple resistance of T. turkestani had reached 35.74 folds after 15 generations. The analysis on the detoxification enzyme activity of different strains showed, compared with the susceptible strains, the carboxyl esterase (CarE), glutathione-s-transferase (GSTs) and the mixed function oxidase (MFO) of the multiple resistance strains had reached 1.21, 1.53 and 9.18 folds, respectively, suggesting the increased activities of CarE, GSTs and MFO could induce the formation of multiple resistance to three insecticides among which MFO might be the main one. By comparing the pesticide sensitivity and detoxification enzyme activity of Mp-R and Ip-R, it was showed that continuous mixing use of the three pesticides might slow the resistance of T. turkestani to fenpropathrin, but accelerate its resistance to abamectin.

WANG Xiao-jun, ZHAO Yi-ying, ZHAO Qian, BAO Jian-hong, ZHANG Yan-na. Multiple resistance selection with insecticides and change of detoxification enzyme activities in Tetranychus turkestani Ugarov et Nikolski (Acari: Tetranychidae).[J]. Chinese Journal of Applied Ecology, 2017, 28(9): 2955-2960.

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