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Chinese Journal of Applied Ecology ›› 2017, Vol. 28 ›› Issue (2): 643-650.doi: 10.13287/j.1001-9332.201702.003

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Degradation of quinclorac by corncob, bamboo charcoal, canola meal adsorption-sodium alginate immobilized Pseudomonas stutzeri PFS-4

ZHANG Ke1*, CHEN Qiang2, CHEN Wei1, CHEN Jia1, GE Sang3, LUO Hong-bing1   

  1. 1College of Civil Engineering, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China;
    2College of Resource Science & Technology, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;
    3School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.

  • Received:2016-05-12 Online:2017-02-18 Published:2017-02-18
  • Contact: * E-mail: zhangke@sicau.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (51278318), the Sichuan Key Scientific Research & Development Program (2013SZ0103,2014NZ0044), and the Scientific Research Innovation Team Project of Sichuan Provincial Education Department (16TD0006).

Abstract: To enhance the degradation of quinclorac in soil and water, corncob, bamboo charcoal, canola meal and sodium alginate were used as complex carriers to immobilize previously isolated bacterial strain Pseudomonas stutzeri PFS-4. Orthogonal experiment was conducted to optimize immobilization condition, and the degradation efficiency of quinclorac by immobilized bacteria and free bacteria in water and soil were further investigated. The results indicated that the optimal conditions for immobilization were: alginate concentration 4%, proportion of adsorption carrier (corncob: bamboo charcoal: canola meal=1:2:1), calcium chloride 3%, crosslinking time 4 h. Immobilized bacteria and free bacteria could effectively degrade 91.4% and 72.8% of quinclorac (800 mg·L-1) after 6 d inoculation at 30 ℃ and pH 7.0. The removal rates of quinclorac by immobilized bacteria and free bacteria were affected in actual wastewater and soil treatments, but the quinclorac removal rate remained at 84.2% in soil and 74.3% in water, respectively. The results also demonstrated that the carrier and entrapment media significantly affected the quinclorac removal in soil, and the degradation rate of quinclorac in soil was significantly positively correlated with turnover frequency. Therefore, the approach of corncob, bamboo charcoal, canola meal adsorption-sodium alginate immobilized P. stutzeri PFS-4 possesses application potential in in situ remediation of quinclorac contaminated water and soil due to its buffering to adverse conditions.