Abstract: In order to provide potentially valuable resource for applying mycorrhizal biotechnology in tea industry, the distribution of AMF resources from Anhui tea area was studied. AMF infection rate, the series of infection, the intensity of infection and the type of mycorrhizae of tea plant were measured using an improved acetic acid ink staining method. AMF spores were isolated by wet screening technique and identified based on morphological characteristics. The results indicated that AMF could invade the root of tea plant and form AMF-plant symbioses, AMF infection rate ranged from 36% to 95%, and the series of infection was above 3 and the intensity of infection was strong. A total of 36 species of AMF belonging to 8 genera in the rhizosphere of tea plant were identified from Anhui tea area. Among them, Funneliformis constrictum was the dominant species; Glomus reticulatum, Acaulospora spinosa, Acaulospora foveata, Acaulospora gerdemannii, Acaulospora bireti-culata and Acaulospora excavata were the common species; F. badium, F. verruculosum, Acaulospora morrowae, F. claroideum, Acaulospora rehmii, Acaulospora cavernata, G. halonatum, Acaulospora denticulata, F. geosporum, G. etunicatum, Acaulospora mellea and Entrophospor infrequens were the rare species, and the other species were the uncommon species. The communities of AMF shared relatively low similarity (0.14-0.55) at different sampling sites which belonged to the low and medium level. Correlation analysis showed that spore density had a positive correlation with the infection rate, and species richness had a positive correlation with the infection rate and T-GRSP. There was typical AM structure in root of tea plant in Anhui tea area and the diversity of AMF was rich. All of these could provide rich resources for the development of special AMF fertilizer for tea plant.