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Chinese Journal of Applied Ecology ›› 2017, Vol. 28 ›› Issue (9): 2984-2992.doi: 10.13287/j.1001-9332.201709.034

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Effects of endophytic bacteria 252 and 254 on peroxidase (POD)and catalase (CAT)activities of wheat seedlings under salt stress.

ZHAO Long-fei1*, XU Ya-jun1, LAI Xin-he2, CHANG Jia-li3, OU Qi-fan1, MENG Jia-qi1, PENG Ding-hua1   

  1. 1Key Laboratory of Plant-Microbe Interactions of Henan Universities, College of Biology and Food Sciences, Shangqiu Normal University, Shangqiu 476000, Henan, China;
    2Institute of Translational Medicine, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China;
    3Division of Water Environment Protection, College of Environment, Tsinghua University, Beijing 100084, China.
  • Received:2016-10-31 Online:2017-09-18 Published:2017-09-18
  • Contact: * E-mail: hnzhaolongfei@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (U1204301), University Key Scientific Research Project of Henan Province (17A180011), National Innovation Experiment Program for University Students of China (20161048302) and Innovation Experiment Program for University Students of Shangqiu Normal University(2016DXS04)

Abstract: Taking wheat variety Zhou18 as test material, salt stress, salt stress plus inoculation of endophytic bacteria, and no treatment (control) were set, and the effects of inoculation of endophytic bacteria on the growth of wheat seedlings under salt stress were investigated through pot experiment. The results showed that endophytic bacterial strains 252 and 254 shared the highest similarity with Bacillus vallismortis and Agrobacterium tumefaciens, respectively. On the 14th day, treatment of endophytic bacterium 252 plus salt stress increased POD activity in wheat seedlings with increasing the salt concentration, and showed the highest POD activity (13370 U·g·min-1) at salt concentration of 100 mmol·L-1. On the 21th day, POD and CAT activities in the treated wheat seedlings were significantly higher than those of controlled group, and reached the maximum on the 28th day under salt concentrations of 150 mmol·L-1 and 250 mmol·L-1, respectively. By contrast, wheat seedlings inoculated with strain 254 showed the highest CAT activity under salt concentration 250 mmol·L-1 on the 14th day. Seedlings treated with strain 254 had significantly higher POD and CAT activities at salt concentration 100-250 mmol·L-1 on the 21th day, and CAT activity was the highest at salt concentration of 150 mmol·L-1. On the other hand, CAT activity peaked at salt concentration of 200 mmol·L-1 on the 28th day. Therefore, the repairing effects of inoculated endophytic bacteria on wheat seedlings varied along with its culture stage and salt concentration. Endophytic bacterial strains 252 and 254 could enhance POD and CAT activities in wheat seedlings under salt stress, and exert significant repairing effects on the growth of wheat seedlings.