Abstract: The otolith of Electrona carlsbergi has diverse morphological characteristics. In order to further study the morphological diversity, a total of 456 sagittal otoliths of E. carlsbergi (standard length 6.0-8.8 cm), which were collected off the South Shetland Islands, were observed and discriminated. The otoliths of E. carlsbergi were classified into four types based on the morphological characteri-stics, and 77 Fourier feature coefficients of four types were analyzed by elliptical Fourier analysis. Based on the pairwise comparison on Fourier feature coefficients of four types, the proportions of pairs of coefficients with significant differences accounted for 61.0% (highest) and 28.6% (lowest) of the total, respectively. Based on the principal component analysis (PCA) of 77 Fourier coefficients, the cumulative contribution rate of first 22 principal components was 76.5%. 17 Fourier feature coefficients were selected to conduct the stepwise discriminant analysis (SDA), and the total discriminated rate was 87.2%. The contours reconstructed by the elliptical Fourier analysis distinctly reflected the differences among the four types of otolith. Four types for E. carlsbergi’s otoliths which appeared on various fish sizes and body masses indicated that the appearance of otolith types was stochastic. The types of otolith were not consistent in the left and right otoliths, indicating different types occurred between both sides of the otoliths. Types I and II were the main forms of E. carlsbergi otoliths, accounting for 72.6% of the total, while types III and IV were the secondary forms, accounting for 27.4% of the total.