Abstract: The stable hydrogen and oxygen isotopes are environmental isotopes, which widely exist in various kinds of water. Their relative abundance variation in water can indicate the water circulation and mechanism of water use in plant. This research selected two major kinds of greening tree species, evergreen coniferous Platycladus orientalis and deciduous broad-leaved Quercus variabilis, in Beijing mountainous area, and the water movement process in soil-plant-atmosphere continuum was investigated by the variation characteristics analysis of stable hydrogen and oxygen isotope compositions in precipitation, soil water, groundwater, plant stem water and leaf water. The results showed that the meteoric water line equation of the study area was δD=7.17δ¹⁸O+1.45 (R²=0.93), and the soil evaporation line equation was δD=3.85δ¹⁸O+1.45 (R²=0.76). A certain degree of evaporation fractionation existed in the processes of rainfall infiltration into soil water. In different seasons, the δD and δ¹⁸O values of precipitation, soil water and spring water had different variation regularity. In rainy season, the mean δD and δ¹⁸O values were in order of precipitation> spring water>soil water, with the precipitation and soil water supplied spring water together; in dry season, the order was precipitation > soil water > spring water, and the precipitation and spring water both contributed to soil water. The δD and δ¹⁸O fitting line equations of stem water of P. orienta-lis and Q. variabilis were respectively δD=5.03δ¹⁸O-30.78 and δD=3.0δ¹⁸O-48.92. The uptake water of Q. variabilis was more enriched than that of P. orientalis, and the depth of Q. variabilis water uptake in soil profile was shallower than P. orientalis. The leaf water isotopic variation of Q. varia-bilis was more sensitive to atmospheric environment, with the kinetic isotopic fractionation of Q. variabilis being more enriched than that of P. orientalis, but they had the same response to variation of environmental condition.