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Chinese Journal of Applied Ecology ›› 2017, Vol. 28 ›› Issue (7): 2361-2368.doi: 10.13287/j.1001-9332.201707.017

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Challenges and probable solutions for using stable isotope techniques to identify plant water sources in karst regions: A review

NIE Yun-peng1,2, CHEN Hong-song1,2*, WANG Ke-lin1,2, SCHWINNING Susanne3   

  1. 1Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
    2Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, Guangxi, China
    3Department of Biology, Texas State University, San Marcos, TX 78666, USA
  • Received:2017-02-24 Revised:2017-04-27 Published:2017-07-18
  • Contact: *mail:hbchs@isa.ac.cn1
  • Supported by:
    This work was supported by the National Key Research and Development Program of China (2016YFC0502402), the National Natural Science Foundation of China (31570428), and the Young Scholars of Western China (for Yunpeng Nie).

Abstract: Karst regions, which account for about 15% of the terrestrial surface area, are characte-rized by specific hydrogeological structure different from most non-karst regions. Thus, many research methods that are used in non-karst regions cannot be directly used in karst regions. This issue is especially relevant to research on plant water sources. In this paper, origins and possible solutions to the common problems associated with research on water sources used by karst plant species were reviewed. Four questions were addressed: 1) why is it important to determine plant water source in karst regions? 2) Why are stable isotopes used? 3) What are the challenges associated with using stable isotopes in karst regions? 4) What are the probable solutions for these challenges? This review emphasized the advantages of using stable isotope techniques to identify sources of water used by karst plant species and the challenges associated with satisfying the prerequisites of this method. It is suggested that sources of water used by plant species in karst regions need not to be divided into specific depths and the method of identifying sources of water used by plant species based on their hydrologic properties was much applicable.