Application of DNA fingerprint in eco-toxicological diagnosis of contaminated soil

Abstract

Abstract: Biomarker can show the exposure-effect relationships at molecular and cellular levels,and is one of the most effective techniques in eco-toxicological diagnosis of contaminated soil.With the progress of molecular biology,a number of selective and sensitive PCR-based DNA fingerprint assays were developed to detect the DNA lesions caused by pollutants.The DNA fingerprint techniques mainly include random amplified polymorphic DNA(RAPD),polymerase chain reaction-single strand conformation polymorphism,amplified fragment length polymorphism,arbitrary primed-polymerase chain reaction,differential display reverse transcription,short-sequence DNA repeat,and restriction fragment length polymorphism.These techniques possess the characters of simplicity,reproducibility and sensitivity,compared with classical methods such as comet assay and micronucleus test.In this paper,the application of three main DNA fingerprints in eco-toxicological diagnosis of contaminated soil was discussed emphatically.

Key words: Gap regeneration, Mixed-broad-leaved Korean pine forests, Tree diversity, Gap structure, Stand dynamics

CLC Number:

QI Xuemei, LI Peijun, LIU Wan, ZHANG Hairong, XU Huaxia, HAN Chunmei. Application of DNA fingerprint in eco-toxicological diagnosis of contaminated soil[J]. cje, 2005, (11): 1351-1356.

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