Screening of cadmium-resistant strains and their effects on cadmium activation

Abstract

Abstract: In this paper,cadmium-resistant bacteria were isolated from heavy metals-contaminated soils through agar plate containing Cd²⁺ as 3CdSO₄·8H₂O.The strains JL-4 and JC-9 were identified as Bacillus sp.and Xanthomonas sp.,respectively,based on their morphological,physical,and biochemical characteristics.In the liquid medium containing Cd²⁺(100 mg·L^-1) and inoculated with JL-4 or JC-9,the available Cd²⁺ content in supernatant was increased by 1 392.3%(JL-4) or 1 410.8%(JC-9),compared with the control,and the pH was decreased to 2.87(JL-4) or 3.18(JC-9).Soil cadmium(100,200 mg·kg^-1) activation experiments showed that the available Cd²⁺ content in soil inoculated with JL-4 or JC-9 was increased by 17.02%～100%(JL-4) or 36.17%～61.80%(JC-9),compared with the control.The optimum temperature for JL-4 and JC-9 was 28 ℃,and the optimum pH for them was 7 and 8,respectively.The two strains could also be resistant to Pb²⁺(800 mg·L^-1),Cu2+(50 mg·L^-1),Ni²⁺(50 mg·L^-1),and Zn²⁺(200 mg·L^-1).

Key words: Forest vegetation, Carbon storage, Heilongjiang Province

CLC Number:

XIA Juanjuan, SHENG Xiafang, JIANG Chunyu. Screening of cadmium-resistant strains and their effects on cadmium activation[J]. cje, 2005, (11): 1357-1360.

References

[1] 龙新宪,杨肖娥,倪吾钟.2002.重金属元素土壤修复技术研究的现状与展望[J].应用生态学报,13(6):757～762.
[2] 东秀珠,蔡妙英.2001.常见细菌系统鉴定手册[M].北京:科学出版社,62～179.
[3] 孙琴,王晓蓉,丁士明.2005.超积累植物吸收重金属的根际效应研究进展[J].生态学杂志,24(1):30～36.
[4] 陈素华,孙铁珩,周启星,等.2002.微生物与重金属的相互作用及其应用研究[J].应用生态学报,13(2):239～242.
[5] 张从,夏立江.2002.污染土壤生物修复技术[M].北京:中国环境科学出版社.
[6] 张利红,李培军,李雪梅,等.2005.镉胁迫对小麦幼苗生长及生理特性的影响[J].生态学杂志,24(4):458～460.
[7] 何池全,李蕾,顾超.2003,重金属污染土壤的湿地生物修复技术[J].生态学杂志,22(5):78～81.
[8] 余贵芬,蒋新,和文样,等.2002.腐殖酸对红壤中铅镉赋存形态及活性的影响[¨.环境科学学报,22(4):508～513.
[9] 郭学军,黄巧云,赵振华,等.2002.微生物对土壤环境中重金属活性的影响[J].应用与环境生物学报,8(1):105～110.
[10] 倪才英,陈英旭,骆永明,等.2004.不同活化剂对铜冶炼厂污染土壤中铜的活化[J].农业环境科学学报,23(1):85～89.
[11] Chanmugathas P, Bollag JM. 1987. Microbial role in immobilization and subsequent mobilization of cadmium in soil suspensions[J]. Soil Sci. Soc. Amer. J., 51:1184～ 1191.
[12] Chanmugathas P, Bollag JM. 1987. Microbial mobilization of cadmium in soil under aerobic and anaerobic conditions[J]. J. Environ. Qual., 16(2): 161 ～ 167.
[13] Eriksson JE. 1989. The influence of pH, soil type and time on adsorption and uptake by plants of cadmium added to the soil [J].Water Air Soil Poll., 48:317～335.
[14] Ledin M, Pederson K, Allard B. 1997. Effects of pH and ionic strength on the adsorption of Cs, Sr, Eu, Zn, Cd and Hg by Pseudomonas putida [J]. Water Air Soil Pollut . , 93: 367～ 381.
[15] Nriagu JO, Pacyna JM. 1988. Quantitative assessment of worldwide contamination of air water and soils by trace metals [J].Nature, 333:134～ 139.
[16] Singh BR, Narwal RP, Jeng AS, et al. 1995. Crop uptake and extract ability of cadmium in soils naturally high in metals at different pH levels[J]. Commun . Soil Sci. Plant Anal., 26:2133～2142.
[17] Tessier A, Campbell PGC, Bisson M. 1979. Sequential extraction procedure for the speciafion of particulate trace metals[J]. Anal.Chem., 51(7) :844～851.