Comparison of the quantity of normal flora in digestive tracks betweeol SPF and ordinary chicken

Abstract

Abstract: Qualitative and quantitive investigations wasere carried out on the intestinal bacterial community in SPF and ordinary chicken.Nine kinds of dominant intestinal bacteria were selected and counted on various selective media.The results showed that when comparing SPF with ordinary chicken,significant differences were found in the number of clostridium,eubacterium,staphylococcus and escherichia coli in crop (P<0.01),staphylococcus(P<0.05) in proventriculus,bacteroides (P<0.01) in ileum,lactobacillus (P<0.05) in caecum,and bifidobacterium and lactobacillus (P<0.05) in rectum.

Key words: Ginger, Meloidogyne incognita, Inoculation density, Yield

CLC Number:

Q938.1

LIU Hongjie, CHENG Liangui, NIU Zhongxiang, WANG Jianchang, NING Haiqiang . Comparison of the quantity of normal flora in digestive tracks betweeol SPF and ordinary chicken[J]. cje, 2005, (7): 795-798.

References

[1] 李影林.1991.培养基手册[M].吉林:吉林科学技术出版社.
[2] 吴仲文,李兰娟,马伟杭,等.2000.肠道微生物定植抗力的新指标--B/E值[J].浙江预防医学,12(7):4～5.
[3] 何昭阳,王增辉,吴延春,等.2000.雏鸡消化道主要正常菌群定植规律的研究[J].畜牧兽医学报,31(1):41～48.
[4] 杨景云.1997.医用微生态学[M].北京:中国医药科技出版社.
[5] 杨洁彬,郭兴华.1996.乳酸菌--生物学基础及应用[M].北京:中国轻工业出版社.
[6] 郭兴华.2002.益生菌基础与应用[M].北京:北京科学技术出版社.
[7] 熊德鑫.1986.厌氧菌分离和鉴定方法[M].南昌:江西科学技术出版社.
[8] 光冈知足.1970.肠道菌の世界[M].东京:东京从文社.
[9] Apajalahti JHA, Sarkilahti LK, Maki BRE, et al. 1998. Effective recovery of bacterial DNA and percent-guanine-plus- cytosinebased analysis of community structure in the gastrointestinal tract of broiler chickens [J]. Appl. Environ. Microb., 64: 4084 ～4088.
[10] Arthur O, Erika I, Seppo S. 2002. The role of the intestinal microflora for the development of the immune system in early childhood [J]. Europ .J. Nutri., 41(32) :37.
[11] Authur H, Beame B, Summers M, et al. 1990. Biological Control of Salmonella Typhimurium in Young Chickens[J]. Avian diseases, 3626 ～ 3633.
[12] Mead GC. 1997. Bacteria in the gastrointestinal tract of birds [J].Gastrointestinal Microb., 54(13): 216～ 240.
[13] Van der Waai JD, Berghuis JM, Lekkerkerk-Van der Wees JEC.1971. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice[J]. J. Hyg., 69: 405.
[14] Van der Wielen PWJJ, Keuzenkamp DA, Lipman LJA, et al.2002. Spatial and temporal variation of the intestinal bacterial community in commercially raised broiler chickens during growth[J]. Microb. Ecol., 44:286～293.
[15] Wierup M, Hopfer RJ. 1988. Epidemiological evaluation of the salmonella controlling effect of a nation wide use of a competitive exclusion culture in poultry [J]. Poult. Sci., 67:1026～1033.