Isolation of biosurfactant-producing microorganisms and their stability

Abstract

Abstract: 52 biosurfactant-producing microorganisms were isolated with the methods of enrichment culture and plate cultivation from oil sludge of Daqing Oil Field.Experiments of oil displacement activity and surface tension showed that the three strains (including B₂₂,B₂₄ and B₂₅) had high surface activity and low surface tension.The analysis of physico-chemical properties indicated that the biosurfactant produced by B₂₂ could bear high temperature up to 120 ℃,while the other two could bear 80 ℃.The three biosurfactants had a wide adaptability to pH.B₂₂ had a wide adaptable pH range of 4.0～13.0,while that of B₂₄ and B₂₅ was 2.0～13.0.NaCl concentration had no obvious effect on the biological activity of the biosurfactants.Employing the 3 strains biosurfactants to treat oil sludge,more than 70% of the oil was removed after 72 h.

Key words: Agricultural landscape, Ecological construction, Soil and water conservation, Remote sensing and GIS

CLC Number:

Q935

NIU Mingfen, LI Fengmei, HAN Xiaori, GUO Shuhai, NIU Zhixin, LENG Yanhui, ZHANG Chungui. Isolation of biosurfactant-producing microorganisms and their stability[J]. cje, 2005, (6): 631-634.

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