Abstract: Based on the redox reaction of special substrates catalyzed by fungal laccase, an effective screening method for isolating laccase-producing macro-fungi strains was designed. Guaiacol and α-naphthol were used for color-developing in determining this enzymatic reaction and as the factors for restrictive selection, and lignin was used as an inducer to enhance the enzymatic productivity. The extracellular laccase-productivity of general macro-fungi and edible mushroom strains preserved in our laboratories or purchased from other institutions and supermarkets was investigated, but none of the strains could effectively produce laccase, or the produced laccase had very low activity. Taking our method as the means for primary isolation, 15 potent laccase-producing macro-fungi strains were successfully isolated from wild woody districts like poplar forest land.Three high-efficient macro-fungi strains, A21, X18 and Y11, were further screened, with the extracellular laccase activities being 255.9, 248.7 and 277.3 U·ml^-1, respectively, which could be used as the strains with considerable potential of industrialization.

Key words: Campylomma chinensis, Lantana camara, Volatiles, Olfactory stimuli, Floral colors, Visual stimuli