Abstract: In this paper, Schima superba populations were collected from different altitudes at Tiantai Mountain in Zhejiang Province, and their genetic diversity and genetic differentiation were analyzed by using inter simple sequence repeat (ISSR) molecular technique. ISSR amplification was conducted with 12 primers for 80 individuals from 4 populations, and 154 polymorphic loci were detected from total 170 loci, with the percentage of polymorphic loci (P) being 90.59%. The Shannon’s information index (I) and Nei’s gene diversity (h) of S. superba at species level were 0.5033 and 0.3408, respectively, indicating a high genetic diversity of S. superba at species level, while the genetic diversity of S. superba at population level was lower than that at species level, with the mean values of P, I and h being 63.68%, 0.3789 and 0.2608, respectively. Molecular variance (AMOVA) analysis demonstrated that the among-population component accounted for 29.56% of the total variation, while the within-population component accounted for 70.44%, the genetic differentiation coefficient (G_st) among the populations being 0.2348. The gene flow among the populations was 1.6293, and the mean pair-wise genetic distance was 0.1500.

Key words: Phyllotreta striolata, Environmental factors, Dispersion, Marking recapture method