Effects of environmental factors on key carbon-nitrogen metabolizing and antioxidizing enzyme activities of Amaranthus

Abstract

Abstract: This paper studied the physiological indexes of Amaranthus cultivated in a big vinyl house in early spring under different combinations of five levels of sunlight intensity,air temperature,soil temperature,carbon dioxide concentration,and nitrogen fertilization.The results showed that for carbon and nitrogen metabolism,nitrate reductase activity (NRA) had significantly positive correlations with sunlight intensity and air temperature at different nitrogen application levels,and the order was A (no net and no film)>B (no net and one layer film)>C (one layer net and one film)>D (two layers net and one film)>E (three layers net and one film).Compared with A (control),the NRA of the other four treatments was decreased by 6.45%,34.21%,61.54% and 63.78% respectively.CO₂ concentration and air and soil temperature affected the activity of PEPC significantly.Of all treatments,the PEPC activity in treatment B was the highest,and that of B,C,D and E was 59.69%,28.57%,-10.71% and -17.86% higher than A,respectively.For the metabolism of active oxygen system,SOD activity was positively correlated with sunlight,but negatively correlated with CO₂ concentration,and the order was A>B>C>D>E.POD activity was negatively correlated with sunlight and air temperature,with the order of A<B<C<D<E,and CAT was positively correlated with sunlight and air temperature,with the order of B>A>C>D>E in all combinations.The enzyme activities of B with 20 kg·667 m^-2,17 800 lx sunlight intensity,21.2 ℃ soil temperature,23.5℃ air temperature and 1 500 μmol CO₂·mol^-1 were the most cooperative,which made the Amaranthus cultivation technical system of "high yield,high efficiency and excellent quality" be possible.

Key words: La(NO3)3, ICP-MS, Micronucleus rate, DNA cleavage, Genetic toxicity

CLC Number:

S636.4

SUI Yihu, ZHU Shidong, ZHANG Zixue, HU Deping, SHU Yingjie. Effects of environmental factors on key carbon-nitrogen metabolizing and antioxidizing enzyme activities of Amaranthus[J]. cje, 2005, (8): 925-929.

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