Abstract: Five sampling sites of mine tailings inhabited by Miscanthus floridulus with the coverage of 0, 17%, 41%, 71%, and 89% were selected to analyze the ynamics of microbial community structure and its relationships with mine tailings’ physical and chemical properties, heavy metals contents, and plant biomass. With the inhabitation of M. floridulus, the mine tailings’ organic carbon, total nitrogen, total phosphorus, NH₄⁺-N, NO₃^--N, and available phosphorus contents, ggregate stability, and maximum waterholding capacity increased significantly, but the total and DTPAextractable heavy metals contents  significantly decreased. The principal component analysis indicated that the microbial community structure changed significantly with the inhabitation of M. floridulus. The Grampositive bacterial fatty acids, Gramnegative bacterial fatty acids, fungal fatty acid, actinomycete fatty acid, mycorrhizal fatty acids (16∶1ω5c and 20∶3ω3), algal fatty acids, and microbial community diversity (ShannonWiener index) all increased significantly and had significant positive correlations with the mine tailings’ total nitrogen, NO₃^--N and available phosphorus contents, aggregate stability, and the aboveground and belowground biomass of M. floridulus. Only the Gramnegative bacterial and mycorrizal phospholipid fatty acids were negatively correlated with mine tailings’ DTPAextractable Cu content.

Key words: Winter wheat, Small amount precision seeding, Yield, Tiller, Physiological function, Metabolism activity, Spike per plant