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cje ›› 2010, Vol. 29 ›› Issue (03): 543-548.

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Genetic diversity of wapiti population in eastern Wandashan Mountains of Heilongjiang Province, China based on microsatellite analysis.

TIAN Xin-min1,2|ZHANG Min-hai1;ZHANG Hui1;YANG Chun-wen2;JIN Zhi-min2   

  1. 1College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China; 2Department of Biology, Mudanjiang Normal University, Mudanjiang 157012, Heilongjiang, China
  • Online:2010-03-10 Published:2010-03-10

Abstract: The eastern Wandashan Mountains of Heilongjiang Province is one of theareas with high population density of wapiti (Cervus elaphus xanthopygus).
In this paper, 167 wapiti fecal samples were collected there in two winters to identify their DNA based on 7 microsatellite loci, aimed to study the genetic div
ersity of the population, and the effects of the population’s dramatic decline in recent years on the population genetic structure. The DNA of the samples was
assigned to 66 individuals. The average numbers of the alleles and effective alleles were 9.00±2.77 and 3.97±0.99, respectively, the average polymorphism information content was 0.69±0.09, and the average observed and expected heterozygosity values were 0.69±0.08 and 0.74±0.08, respectively. The population was significantly deviated from Hardy-Weinberg Equilibrium, but the inbreeding coefficient was 0.060, not significantly deviated from zero. It was suggested that the genetic diversity of the population was still higher, and the dramatic decline of the population didn’t yet affect the population genetic structure due to time lag.

Key words: Myzus persicae, Imidacloprid, Low sublethal sprays, Bioassay, Time-concentration-mortality modeling