Abstract: Atrazine is one of the most widely used herbicides in China. In this paper, Eriocheir sinensis was treated with 0.01, 0.1, and 1 mg·L^-1 of atrazine for 90 days, and single-cell gel electrophoresis was used to study the impacts of atrazine chronic poisoning on the hemolymph DNA of E. sinensis. The fluorescence microscope observation and the CASP analysis of the images showed that all the three concentrations of atrazine could induced the DNA damage of E. sinensis hemolymph. With the increase of atrazine concentration, the TM value and the percentage of TailDNA increased gradually, and the impacts were most significant when the concentration of atrazine was 1 mg·L^-1, suggesting that definite concentration atrazine could induce the broken of DNA chain, and destroy the DNA structure of E. sinensis hemolymph.

Key words: Caragana intermedia Rhizobia, Diversity, Resistance