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cje ›› 2010, Vol. 29 ›› Issue (07): 1340-1346.

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Effects of citric acid and oxalic acid on the growth and physiology of Iris lactea var. chinensis  under Pb stress.

WANG Hong-yan, TONG Hai-ying, HUANG Su-zhen, YUAN Hai-yan   

  1. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing Botanical Garden, Nanjing 210014,China
  • Online:2010-07-08 Published:2010-07-08

Abstract: A hydroponic experiment was conducted to study the effects of exogenous citric acid (0.5 and 5 mmol·L-1) and oxalic acid (0.5 and 5 mmol·L-1) on the growth and physiology of Iris lactea var. chinensis  under 100 and 500 mg·L-1 Pb stress. Comparing with Pb stress alone, adding 5 mmol·L-1of citric acid promoted the shoot growth of I. lactea var. chinensis significantly. The addition of oxalic acid mitigated the impact of 500 mg·L-1Pb stress on the root growth of I. lactea  var. chinensis. Adding 0.5 mmol·L-1of oxalic acid alleviated the 500 mg·L-1Pb stress on the shoot growth, and increased the root dry mass significantly under 100 and 500 mg·L-1 Pb stress. The SOD activity in I. lactea var. chinensis  leaves under 100 mg·L-1Pb stress was increased significantly by adding 0.5 mmol·L-1of citric acid, and the POD activity of  I. lactea var. chinensis under 100 mg·L-1 Pb stress was increased significantly by adding 0.5 and 5 mmol·L-1 of citric acid or 0.5 mmol·L-1of oxalic acid. The impact of 500 mg·L-1 Pb stress on the photosynthetic pigments of I. lactea var. chinensis was alleviated by the addition of 5mmol·L-1 of citric acid or 0.5 mmol·L-1of oxalic acid. Adding 0.5 and 5 mmol·L-1of citric acid or 0.5 mmol·L-1 of oxalic acid reduced the membrane damage of I. lactea var. chinensis leaves under 100 and 500 mg·L-1 Pb stress.

Key words: Urban forest, Correlation, Growth index, Compounding