Soil macroarthropods, an important component of ecosystems, play a key role in maintaining ecosystem structure and functioning. Here, we investigated soil macroarthropod community composition by hand sorting method to examine the effects of fire disturbance on soil macroarthropod at sites treated with burning and burning + reclamation, and a natural forest in karst of south Guizhou in October of 2015 and January, April and July of 2016. A total of 214 individuals were captured, belonging to five classes, 15 orders, 38 families and 54 groups. Scarabaeidae larvae were the dominant group of the soil macroarthropod community. The results from the principal component analysis showed that the community structure of soil macroarthropod differed obviously under different treatments. There were no significant differences in the group number, density, Shannon index and the density of each functional group of soil macroarthropod among the different treatments. Fire had significant impacts on seasonal variations of group number, density, Shannon index and the density of omnivore group. Seasonal variations of predator group were observed under different treatments. Soil available phosphorus concentration was significantly correlated with Shannon index of soil macroarthropod community. Our results indicated that different disturbances had different effects on seasonal variations of soil macroarthropod, which is possibly related to disturbance type and recovery time of burned areas.