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cje ›› 2010, Vol. 29 ›› Issue (10): 1967-1972.

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Allozyme analysis on genetic diversity of eight geographical populations of Oedaleus asiaticus in Inner Mongolia.

GAO Shu-jing1, LI Dong-wei2, LIU Ai-ping1, YAN Zhi-jian1, CHANG Xiu-qing3   

  1. 1Grassland Research Institute of Chinese Academy of Agricultural Sciences, Hohhot 010010, China|2Institute of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China|3Station of Plant Protection of Agricultural Bureau in Siziwang County, Wulanchabu 011800, Inner Mongolia, China
  • Online:2010-10-08 Published:2010-10-08

Abstract: By using polyacrylamide gel electrophoresis of allozymes, this paper studied the genetic diversity among and within eight geographic populations of Oedaleus asiaticus in Inner Mongolia. Eight allozymes, including malate dehydrogenase (MDH), malic enzyme (ME), alcohol dehydrogenase (ADH), glutamate dehydrogenase (GDH), glutamic-oxaloacetic transaminase (GOT), isocitrate dehydrogenase (IDH), adenylatese (AK), and hexokinase (HK), were analyzed. Fourteen loci and twenty-five alleles were detected, with 7 loci polymorphic, and the overallproportion of polymorphic loci (P) was 42.86%. The average number of the alleles per locus (A) was 1.786, the expected heterozygosity He was 0.072, and the mean genetic distance was 0.069-0.235. The dendrogram was constructed by using UPGMA method, and the results indicated that the genetic distances were correlated with the geographical distances among the populations. Among the eight geographic populations of O. asiaticus, the coefficient of gene differentiation Fst was 0.086, and the number of migrants per generation (Nm) was 3.142. Our study indicated that there existed certain degree of genetic differentiation and gene flow among the eight geographic populations of O. asiaticus in Inner Mongolia.

Key words: Plutelia zylostella, Cruciferous vegetable, Experimental population life table