Abstract: Aimed to clarify the toxicological mechanism of bisphenol A (BPA) in aquatic animal, the toxicity and histological damage of BPA on zebrafish (Danio rerio) were studied. BPA had acute toxicity on zebrafish. The 96 h LC₅₀of BPA was 6.3 mg·L^-1. After 96 h exposure to BPA, the liver damage  were observed in concentration-dependent way. In high BPA group, the testis structure was damaged, and the atresia rate of ovarian follicle was 35%. In 0.2 mg BPA·L^-1 group, liver was slightly damaged, gonad had no morphological change, the rate of gametes number in late phase Ⅲ to that in phase Ⅳ in ovary had some increase, and oocyte size increased significantly (P<0.05). In 2 mg BPA·L^-1group, liver was damaged, hepatocyte cloudy swelling and pyknosis were observed, and the rate of gametes number in late phase Ⅲ to that in phase Ⅳ in ovary was slightly increased. In 6.1 mg BPA·L^-1 group, liver was badly damaged with cell vacuolization, necrosis, and cavity, testis structure was subject to certain damage, pyknotic nuclei were also observed, and atresia of overian follicle increased significantly. The results indicated that liver was the target organ of BPA. It remains to be further studied whether gonad damage is caused by toxicological influence or by endocrine disrupting.

Key words: Erythroneura sudra, Erigonidum graminicola, Three-dimentional distribution pattern, Time series dynamics