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cje ›› 2011, Vol. 30 ›› Issue (09): 2002-2006.

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Genetic diversity of two wild  Penaeus monodon broodstock populations.

TAN Shu-hua1,2, WANG Gui-zhong 2**, LI Shao-jing2   

  1. 1 School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, Hunan, China; 2 College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, Fujian, China
  • Online:2011-09-08 Published:2011-09-08

Abstract: By using RAPD and mtDNA 16S rRNA gene sequence analysis, this paper studied the genetic diversity and differentiation of two Penaeus monodon wild broodstock populations collected from Hainan and Malaysia. Using 15 random primers, a total of 82 RAPD loci were detected. For the Hainan and Malaysia populations, the proportion of polymorphic loci was 75.90% and 76.83%, heterozygosity was 0.199 and 0.218, and genetic diversity index was 0.276 and 0.288, respectively, and the genetic distance between the two populations was 0.015. The genetic variation revealed by 16S rRNA gene sequence analysis was low. he nucleotide diversity of the Hainan and Malaysia populations was 0.011 and 0,respectively, and the genetic distance between the two populations was 0.008. Phylogeographic pattern analysis indicated that Malaysia population had a higher genetic diversity than Hainan population, and the Hainan population could be derived from Malaysia population. Therefore, the broodstock imported from Malaysia could be used as the founder in genetic breeding programs.

Key words: Hangzhou, West Lake, Water body, Heterotrophic bacteria, Restricting factors