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cje ›› 2005, Vol. ›› Issue (1): 6-8,96.

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Screening of copper-resistance strains and their biological characters

JIANG Chunyu, SHENG Xiafang, XIA Juanjuan   

  1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2003-10-12 Revised:2003-12-12 Online:2005-01-10

Abstract: Copper-resistance (Cu 200 mg·L-1) strains B and G were screened out from 42 soil samples,which were primarily identified as Achromobacter and Bacillus.Strain B and G were inoculated to the solution containing Cu (150 mg·L-1 ) for activation tests.The results showed that in the liquid medium inoculated strain B and G,the contents of Cu2+ increased by 475.5% and 249.5% respectively compared with the control,and pH decreased from 6.5 to 4.2 and 5.0,respectively.The activation efficiency of the bacteria correlated to their ability to produce acid.The optimum growth conditions for them were: temperature 28 ℃ for both strain B and G,pH 6~7 for strain B and 8 for strain G.Strain G grew well in the medium containing 5% NaCl.Moreover,strain B and G can also be resistant to Pb2+ (400 mg·L-1 ) and Cd 2+ (100 mg·L-1).

Key words: Biosafety, Transgene escape, Ecological risk, Biodiversity

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