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cje ›› 2011, Vol. 30 ›› Issue (03): 430-437.

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Community structure of denitrifying bacteria in a hybrid AS-biofilm process under aerobic condition.

FANG Fang, WANG Shu-mei, FENG Cui-jie, CHEN Shao-hua   

  1. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, Fujian, China
  • Online:2011-03-08 Published:2011-03-08

Abstract: By using nirK and nirS as the markers, and with the methods of PCR and denaturing gradient gel electrophoresis (DGGE), this paper studied the community structure of denitrifying bacteria in a hybrid AS-biofilm reactor under aerobic condition. In the samples collected, most of nirK-containing denitrifiers were belonged to the known Rhizobiales bacteria of α-proteobacteria, while the nirS-containing denitrifiers were similar to β-proteobacterium (Rhodocyclales and Burkholderiales) and γ-proteobacterium (Pseudomonadales). Some specific sequences were found in nirS- and nirK-denitrifying communities. The data also demonstrated that the growth patterns of bacteria in biofilm or activated sludge could make a significant shift on the denitrifying community structure. These results would provide fundamental information for the understanding of the denitrificaiton in the hybrid AS-biofilm process under aerobic condition.