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cje ›› 1994, Vol. ›› Issue (1): 51-56.

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Allelopathic Effects and Preliminary Isolation of Root Exudates of Wedelia chinensis(Osb.)Merr

Zeng Rensen1, Ling Xianglian1, Tan Huifen2, Zeng Qiang2   

  1. 1. Agroecology Laboratory, South China Agricultural University Guangzhou 510642;
    2. Institute of Elemento Organic Chemistry, Nankai University, Tianjin 300071
  • Received:1993-05-03 Revised:1993-11-08 Online:1994-02-10

Abstract: Chinese Wedelia(Wedelia chinensis(Osb.)Merr.),a common garden herb species in South china,tends to form a pure community. Its root exudates collected by sand culture shows allelopathic effects on seedling growth of turnip(Raphanus sativus),rice(Oryza sativa).cucumber(Cucumis sativus)and mungbean(Phaseolus aureus).Theinhibitory concentrations of exudates vvere determined.The hydrophobic root exudates of W.chinensis,obsorbed by XAD-4 resin in hydroponic culture system,shows allelopathic effects on the seedling growth of barnyardgrass(Echinoloa crusgalli),lettuce(Laotuca sativa)nd trunip.By means of extraction using petroleum ether, chloroform and methanol,the exudates are separated into three portions:Ra(5.9%),Rb(74.5%)and Rc(19.6%).Rb,most inhibitory porticn,was isolated into 8 fractions by means of preparatory thin layer chromatography Fraction 1 and 32 were most inhibitory.

Key words: nickel, Pseudodiaptomous annandalei, suppression subtractive hybridization (SSH), ferritin.